当前位置：
X-MOL 学术
›
Indian J. Med. Microbiol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Prevalence of oxacillin-susceptible methicillin-resistant Staphylococcus aureus nasal carriage and their clonal diversity among patients attending public health-care facilities.
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-04-01 , DOI: 10.4103/ijmm.ijmm_20_157 Aline Pecanha Muzy Dias 1 , Lorrayne Cardoso Guimarães 2 , Livia B D V Petrucci 3 , Jéssica A Z Pinheiro 3 , Marcos Gabriel Pinheiro 4 , Felipe Rodrigues E Silva 3 , Helvécio C C Póvoa 3 , Fábio Aguiar-Alves 1
Context: Nosocomial infections arise from many microorganisms, including Staphylococcus aureus. Aims: The aim of this study is to determine the molecular epidemiology of circulating methicillin-resistant S. aureus (MRSA) clones among patients attending community and health-care facilities in Nova Friburgo, RJ, Brazil. Methods: A total of 1002 nasal swab samples were collected from May 2010 to September 2015. S. aureus isolates were identified through phenotypic tests, submitted to antimicrobial susceptibility tests and genotypic analysis to detect mecA, panton-valentine leucocidin (PVL) genes, SCCmec, SPA and multilocus sequencing typing (MLST) typing. Results: We identified 294 (29.3%) isolates as S. aureus and 91 (9.1%) as MRSA. A total of 17 isolates did not present a correlation between phenotypic and genotypic resistance profiles. Among MRSA isolates, 17 (18.7%) carried PVL genes. A total of 20 different SPA types were determined, being grouped by MLST into eight different sequence types. ST5/t002 was the most prevalent genotype found among these isolates. Conclusions: There is a gradual colonisation shift happening in the infection pattern by S. aureus in Brazil. The Brazilian Epidemic Clone (ST239-SCCmec IIIa-PVL-) seems to be substituted by isolates from different clonal complexes, such as ST5, ST8 and ST30. The non-correlation between phenotypic/genotypic resistance profile observed in some isolates suggests the presence of other methicillin resistance mechanisms different from mecA presence or a difference in the nucleotide sequence, which prevents the primers to identify the specific region during polymerase chain reaction reactions. MRSA identification should be based on phenotypic and genotypic testing to ensure the various types of resistance mechanisms.
中文翻译：
公共卫生保健机构就诊的患者中苯唑西林敏感、耐甲氧西林金黄色葡萄球菌鼻腔携带的患病率及其克隆多样性。
背景：医院感染由许多微生物引起，包括金黄色葡萄球菌。目的：本研究的目的是确定巴西罗杰斯州新弗里堡社区和医疗机构就诊的患者中循环的耐甲氧西林金黄色葡萄球菌(MRSA) 克隆的分子流行病学。方法：收集2010年5月至2015年9月鼻拭子样本1002份，通过表型检测鉴定金黄色葡萄球菌分离株，进行药敏试验和基因型分析，检测mecA、 panton-valentine leuucocidin（PVL）基因、SCC mec、 SPA 和多位点测序分型 (MLST) 分型。结果：我们鉴定出 294 株 (29.3%) 分离株为金黄色葡萄球菌，91 株 (9.1%) 为 MRSA。总共 17 个分离株没有表现出表型和基因型耐药谱之间的相关性。在 MRSA 分离株中，17 株（18.7%）携带 PVL 基因。总共确定了 20 种不同的 SPA 类型，并通过 MLST 分为八种不同的序列类型。 ST5/t002 是这些分离株中最常见的基因型。结论：巴西金黄色葡萄球菌的感染模式正在逐渐发生定植转变。巴西流行性克隆 (ST239-SCC mec IIIa-PVL-) 似乎被不同克隆复合体的分离株所取代，例如 ST5、ST8 和 ST30。 在一些分离株中观察到的表型/基因型抗性谱之间的不相关性表明存在与mecA不同的其他甲氧西林抗性机制的存在或核苷酸序列的差异，这阻止引物在聚合酶链反应反应期间识别特定区域。 MRSA鉴定应基于表型和基因型检测，以确保各类耐药机制。
更新日期：2020-04-01
Indian Journal of Medical Microbiology ( IF 1.4 ) Pub Date : 2020-04-01 , DOI: 10.4103/ijmm.ijmm_20_157 Aline Pecanha Muzy Dias 1 , Lorrayne Cardoso Guimarães 2 , Livia B D V Petrucci 3 , Jéssica A Z Pinheiro 3 , Marcos Gabriel Pinheiro 4 , Felipe Rodrigues E Silva 3 , Helvécio C C Póvoa 3 , Fábio Aguiar-Alves 1
Affiliation
Context: Nosocomial infections arise from many microorganisms, including Staphylococcus aureus. Aims: The aim of this study is to determine the molecular epidemiology of circulating methicillin-resistant S. aureus (MRSA) clones among patients attending community and health-care facilities in Nova Friburgo, RJ, Brazil. Methods: A total of 1002 nasal swab samples were collected from May 2010 to September 2015. S. aureus isolates were identified through phenotypic tests, submitted to antimicrobial susceptibility tests and genotypic analysis to detect mecA, panton-valentine leucocidin (PVL) genes, SCCmec, SPA and multilocus sequencing typing (MLST) typing. Results: We identified 294 (29.3%) isolates as S. aureus and 91 (9.1%) as MRSA. A total of 17 isolates did not present a correlation between phenotypic and genotypic resistance profiles. Among MRSA isolates, 17 (18.7%) carried PVL genes. A total of 20 different SPA types were determined, being grouped by MLST into eight different sequence types. ST5/t002 was the most prevalent genotype found among these isolates. Conclusions: There is a gradual colonisation shift happening in the infection pattern by S. aureus in Brazil. The Brazilian Epidemic Clone (ST239-SCCmec IIIa-PVL-) seems to be substituted by isolates from different clonal complexes, such as ST5, ST8 and ST30. The non-correlation between phenotypic/genotypic resistance profile observed in some isolates suggests the presence of other methicillin resistance mechanisms different from mecA presence or a difference in the nucleotide sequence, which prevents the primers to identify the specific region during polymerase chain reaction reactions. MRSA identification should be based on phenotypic and genotypic testing to ensure the various types of resistance mechanisms.
中文翻译：
公共卫生保健机构就诊的患者中苯唑西林敏感、耐甲氧西林金黄色葡萄球菌鼻腔携带的患病率及其克隆多样性。
背景：医院感染由许多微生物引起，包括金黄色葡萄球菌。目的：本研究的目的是确定巴西罗杰斯州新弗里堡社区和医疗机构就诊的患者中循环的耐甲氧西林金黄色葡萄球菌(MRSA) 克隆的分子流行病学。方法：收集2010年5月至2015年9月鼻拭子样本1002份，通过表型检测鉴定金黄色葡萄球菌分离株，进行药敏试验和基因型分析，检测mecA、 panton-valentine leuucocidin（PVL）基因、SCC mec、 SPA 和多位点测序分型 (MLST) 分型。结果：我们鉴定出 294 株 (29.3%) 分离株为金黄色葡萄球菌，91 株 (9.1%) 为 MRSA。总共 17 个分离株没有表现出表型和基因型耐药谱之间的相关性。在 MRSA 分离株中，17 株（18.7%）携带 PVL 基因。总共确定了 20 种不同的 SPA 类型，并通过 MLST 分为八种不同的序列类型。 ST5/t002 是这些分离株中最常见的基因型。结论：巴西金黄色葡萄球菌的感染模式正在逐渐发生定植转变。巴西流行性克隆 (ST239-SCC mec IIIa-PVL-) 似乎被不同克隆复合体的分离株所取代，例如 ST5、ST8 和 ST30。 在一些分离株中观察到的表型/基因型抗性谱之间的不相关性表明存在与mecA不同的其他甲氧西林抗性机制的存在或核苷酸序列的差异，这阻止引物在聚合酶链反应反应期间识别特定区域。 MRSA鉴定应基于表型和基因型检测，以确保各类耐药机制。
京公网安备 11010802027423号