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100th Anniversary of Macromolecular Science Viewpoint: Enabling Advances in Fluorescence Microscopy Techniques
ACS Macro Letters ( IF 5.1 ) Pub Date : 2020-08-28 , DOI: 10.1021/acsmacrolett.0c00506 Zhe Qiang 1 , Muzhou Wang 2
ACS Macro Letters ( IF 5.1 ) Pub Date : 2020-08-28 , DOI: 10.1021/acsmacrolett.0c00506 Zhe Qiang 1 , Muzhou Wang 2
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In the past few decades there has been a revolution in the field of optical microscopy with emerging capabilities such as super-resolution and single-molecule fluorescence techniques. Combined with the classical advantages of fluorescence imaging, such as chemical labeling specificity, and noninvasive sample preparation and imaging, these methods have enabled significant advances in our polymer community. This Viewpoint discusses several of these capabilities and how they can uniquely offer information where other characterization techniques are limited. Several examples are highlighted that demonstrate the ability of fluorescence microscopy to understand key questions in polymer science such as single-molecule diffusion and orientation, 3D nanostructural morphology, and interfacial and multicomponent dynamics. Finally, we briefly discuss opportunities for further advances in techniques that may allow them to make an even greater contribution in polymer science.
中文翻译：
大分子科学观点100周年：促进荧光显微技术的进步
在过去的几十年里，光学显微镜领域发生了一场革命，具有新兴的能力，如超分辨率和单分子荧光技术。结合荧光成像的经典优势，如化学标记特异性、无创样品制备和成像，这些方法使我们的聚合物界取得了重大进展。本观点讨论了其中的几种功能，以及它们如何在其他表征技术有限的情况下独特地提供信息。重点介绍了几个例子，证明了荧光显微镜能够理解聚合物科学中的关键问题，例如单分子扩散和取向、3D 纳米结构形态以及界面和多组分动力学。最后，
更新日期：2020-09-15
中文翻译：
大分子科学观点100周年：促进荧光显微技术的进步
在过去的几十年里，光学显微镜领域发生了一场革命，具有新兴的能力，如超分辨率和单分子荧光技术。结合荧光成像的经典优势，如化学标记特异性、无创样品制备和成像，这些方法使我们的聚合物界取得了重大进展。本观点讨论了其中的几种功能，以及它们如何在其他表征技术有限的情况下独特地提供信息。重点介绍了几个例子，证明了荧光显微镜能够理解聚合物科学中的关键问题，例如单分子扩散和取向、3D 纳米结构形态以及界面和多组分动力学。最后，
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