The in vitro regeneration frequency was commonly low, and the quality of adventitious buds was poor, when the traditional methods were used to induce adventitious buds from leaf explants of Jatropha curcas L. through the medium containing low concentration of thidiazuron (TDZ). When treated with high concentrations (6.5–104 mg/L) of TDZ for a certain time (7–120 min), the regeneration frequency and quality of adventitious buds were significantly increased. The best induction rate of adventitious buds (78.58%) and the average number of buds per explant (7.26) were obtained by soaking leaf explants in 26 mg/L TDZ solution for 30 min and then transferring them onto MS medium without hormone for 30 days. Furthermore, the adventitious bud regeneration was affected obviously by source of explants, sterilization time, illumination time and genotype. The subsequent elongation and rooting experiments showed that adventitious bud elongation could be improved by adding diethyl aminoethyl hexanoate (DA‐6) in the medium, and the best elongation effect could be gained when the concentration of DA‐6 was 1.2 mg/L. The elongated shoots induced the roots and developed into small intact plants on the MS medium with 1.5 mg/L sodium nitroprusside (SNP). After domestication, these small plants could be transplanted into the soil and growing normally. Therefore, the method established in this study was helpful to improve the plant regeneration efficiency of leaf explants in J. curcas.

中文翻译：

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不同因子对麻疯树叶片外植体再生的影响。 

当采用传统方法从麻疯树的叶片外植体诱导不定芽时，其体外再生频率通常较低，不定芽的质量也较差。L.通过含有低浓度的噻唑隆（TDZ）的培养基。当用高浓度（6.5–104 mg / L）的TDZ处理一定时间（7-120分钟）时，不定芽的再生频率和质量显着提高。不定芽的最佳诱导率（78.58％）和每个外植体的平均芽数（7.26）是通过将叶片外植体浸入26 mg / L TDZ溶液中30分钟，然后将其转移至不含激素的MS培养基上30天而获得的。 。此外，不定芽的再生受到外植体来源，灭菌时间，光照时间和基因型的明显影响。随后的伸长和生根实验表明，在培养基中添加二乙基氨基乙基己酸二乙酯（DA-6）可以改善不定芽的伸长，当DA-6的浓度为1.2 mg / L时，可获得最佳的伸长效果。细长的芽诱导根，并在含有1.5 mg / L硝普钠（SNP）的MS培养基上发育成完整的小植株。驯化后，这些小植物可以移植到土壤中并正常生长。因此，本研究建立的方法有助于提高叶片外植体的植株再生效率。J.库尔卡斯。