An 8‐week feeding experiment was conducted to investigate the effects of dietary lipid sources on growth performance, digestive capacity, antioxidant capacity and expression of immune‐related genes in the hybrid grouper (Epinephelus fuscoguttatus ♀× E. lanceolatus ♂). Five isoproteic and isolipidic experimental diets were formulated containing fish oil (FO), soybean oil (SO), linseed oil (LO), rapeseed oil (RO) and peanut oil (PO), respectively. Results showed that fish fed the FO diet had the highest weight gain and specific growth rate and the lowest feed conversion ratio. The hepatic aspartate aminotransferase and alanine transaminase activities in the FO group were significantly higher than those in the PO group (p < .05), and the highest total antioxidant capacity was observed in the FO group. Intestinal lipase, trypsin and amylase activities in the LO group were significantly higher than those in the FO and SO groups (p < .05). The mRNA expression levels of Toll‐like receptor 3, Toll‐like receptor 22 and myeloid differentiation factor 88 in the liver of the PO group were significantly elevated (p < .05), while the mRNA expression of pro‐inflammatory cytokines (tumour necrosis factor‐α and interleukin‐1β) in the RO group was significantly higher than that in the FO group, and the highest mRNA expression of anti‐inflammatory cytokines (interleukin‐10 and transforming growth factor‐β1) was recorded in the FO group. In summary, the FO and LO groups showed better growth performance, while the poor growth performance of the PO group could be attributed to aggravation of the inflammatory response. Finally, LO was recommended as the suitable lipid source replacement for FO.

中文翻译：

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日粮植物油对石斑鱼（Epinephelus fuscoguttatus♀×E. lanceolatus growth）生长性能，消化能力，抗氧化能力和免疫相关基因表达的影响

进行了一个为期8周的喂养实验，以研究膳食脂质来源对杂种石斑鱼（Epinephelus fuscoguttatus♀ × E. lanceolatus growth）的生长性能，消化能力，抗氧化能力和免疫相关基因表达的影响。配制了五种等蛋白和等脂实验日粮，分别含有鱼油（FO），大豆油（SO），亚麻子油（LO），菜籽油（RO）和花生油（PO）。结果表明，饲喂FO日粮的鱼增重和比生长率最高，饲料转化率最低。FO组的肝天冬氨酸转氨酶和丙氨酸转氨酶活性明显高于PO组（p <.05），并且在FO组中观察到最高的总抗氧化能力。LO组的肠道脂肪酶，胰蛋白酶和淀粉酶活性显着高于FO和SO组（p  <.05）。PO组肝脏中Toll样受体3，Toll样受体22和髓系分化因子88的mRNA表达水平显着升高（p <.05），而RO组促炎细胞因子（肿瘤坏死因子-α和白细胞介素-1β）的mRNA表达显着高于FO组，而抗炎细胞因子的mRNA表达最高（ FO组记录了白细胞介素10和转化生长因子β1。总之，FO和LO组显示出更好的生长性能，而PO组的生长性能较差可归因于炎症反应的加剧。最后，推荐LO作为FO的合适脂质来源替代。