A 115‐day feeding trial and subsequently a 10‐day challenge test with Vibrio parahaemolyticus were conducted to investigate the effects of dietary antimicrobial peptide APSH‐07 on growth performance, immune response, antioxidative status and vibriosis resistance of abalone Haliotis discus hannai (initial body weight: 2.06 ± 0.01 g; initial shell length: 25.42 ± 0.18 mm). Four artificial diets were designed with 0 (artificial diet control), 7.5, 15.0 and 22.5 mg/kg of APSH‐07, respectively. The brown alga Laminaria japonica was used as the live food control. Results showed that the specific growth rates of abalone in the groups with 7.5 and 15.0 mg/kg dietary APSH‐07 were significantly higher than those in the controls (p < .05). The total haemocyte counts and respiratory burst activity in haemolymph of abalone in the group with 7.5 mg/kg of dietary APSH‐07 were significantly higher than those in the groups with 0 and 22.5 mg/kg of dietary APSH‐07 (p < .05). The gene expression levels of Mn‐superoxide dismutase and thioredoxin peroxidase 2, and the activities of glutathione peroxidase in the group with 7.5 mg/kg of dietary APSH‐07 were significantly higher than those in the other groups (p < .05). Cumulative mortality of abalone after the challenge test was significantly decreased in the group with 7.5 mg/kg of dietary APSH‐07 supplementation. Supplementation of 22.5 mg/kg dietary APSH‐07 significantly increased the cumulative mortality. In conclusion, 7.5 mg/kg of dietary APSH‐07 supplementation had the better growth performance, higher antioxidation, immune and disease resistance capacity of abalone. Excessive supplementation of dietary antimicrobial peptide APSH‐07 (22.5 mg/kg) had significantly negative effects. Further studies are needed to determine the optimal level of dietary APSH‐07 supplementation for abalone.

中文翻译：

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日粮抗菌肽APSH‐07对鲍氏鲍氏鲍鱼生长性能，免疫反应和抗弧菌性的影响

进行了115天的喂养试验以及随后的10天副溶血性弧菌挑战试验，以研究膳食抗菌肽APSH-07对鲍鱼拟南芥（Haiotis discus hannai）初始生长性能，免疫反应，抗氧化状态和抗弧菌性的影响重量：2.06±0.01 g；初始外壳长度：25.42±0.18 mm）。设计了四种人工饮食，分别采用0（人工饮食控制），7.5、15.0和22.5 mg / kg的APSH‐07。褐藻海带被用作活食的控制。结果表明，7.5和15.0 mg / kg日粮APSH‐07组的鲍鱼比生长速率显着高于对照组（p <.05）。饮食7.5 mg / kg的饮食APSH‐07组的鲍鱼的总血细胞计数和呼吸爆发活性显着高于饮食水平为0和22.5 mg / kg的饮食APSH‐07的组（p  <.05 ）。日粮7.5 mg / kg的APSH‐07组中Mn超氧化物歧化酶和硫氧还蛋白过氧化物酶2的基因表达水平以及谷胱甘肽过氧化物酶的活性显着高于其他组（p <.05）。补充7.5 mg / kg饮食APSH-07的组中，攻击试验后鲍鱼的累积死亡率显着降低。补充22.5 mg / kg的饮食APSH‐07可显着增加累积死亡率。总之，添加7.5 mg / kg的日粮APSH‐07具有更好的鲍鱼生长性能，更高的抗氧化，免疫和抗病能力。饮食中过量添加抗菌肽APSH-07（22.5 mg / kg）具有明显的负面影响。需要进一步的研究以确定鲍鱼APSH-07膳食的最佳添加水平。