A 56‐day feeding trial was conducted to evaluate the effects on the growth performance, digestive enzyme activity, inflammatory genes expression and intestine histology of silver sillago, Sillago sihama (Forsskål 1775), by replacing fish meal (FM) with low‐gossypol cottonseed meal (LCSM). Five isonitrogenous and isolipidic diets were formulated, including R0 group (control, containing 550.0 g/kg FM), R16 group (88.5 g/kg LCSM and 461.5 g/kg FM), R32 group (177.0 g/kg LCSM and 373.0 g/kg FM), R48 group (265.5 g/kg LCSM and 284.5 g/kg FM) and R64 group (354.0 g/kg LCSM and 196.0 g/kg FM). Fish fed R0 and R16 groups had a significantly higher weight gain rate (WGR) and specific growth rate (SGR) than R48 and R64 groups (p < .05). In contrast to whole‐body crude protein, whole‐body moisture increased with the FM level of substitution (p < .05). With the increased amount of LCSM in the diet, the activity of intestinal amylase (AMS) increased significantly (p < .05), and intestinal trypsin (TRP) decreased (p < .05). Dietary LCSM substitution upregulated the expression of intestinal tumour necrosis factor‐α (TNF‐α), the nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐κB), and interleukin one beta (IL‐1β), but downregulated tight junction proteins ZO‐1(ZO‐1), transforming growth factor beta‐3 (TGF‐β3) and interleukin 10 (IL‐10) expression. Histological analysis revealed progressive morphological damage to the mid‐intestine with higher levels of FM replacement. These results showed that 88.5 g/kg (16%) of FM replaced by LCSM with amino acids (methionine and lysine) supplementation did not significantly reduce growth compared with FM‐based control.

中文翻译：

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低棉酚棉籽粉替代鱼粉对银新罗鱼（Sillago sihamaForsskál）的生长性能，消化酶活性，肠道组织学和炎症基因表达的影响（1775年）

进行了一项为期56天的饲养试验，以用低棉酚棉籽代替鱼粉（FM）来评估其对西罗戈（Sillago sihama）（Forsskål1775）的生长性能，消化酶活性，炎性基因表达和肠道组织学的影响餐（LCSM）。配制了五种等氮和低脂饮食，包括R0组（对照组，含550.0 g / kg FM），R16组（88.5 g / kg LCSM和461.5 g / kg FM），R32组（177.0 g / kg LCSM和373.0 g / kg）。 kg FM），R48组（265.5 g / kg LCSM和284.5 g / kg FM）和R64组（354.0 g / kg LCSM和196.0 g / kg FM）。饲喂R0和R16组的鱼的增重率（WGR）和比生长率（SGR）明显高于R48和R64组（p <.05）。与全身粗蛋白相反，全身水分随FM取代水平的增加而增加（p  <.05）。随着饮食中LCSM量的增加，肠淀粉酶（AMS）的活性显着增加（p  <.05），肠胰蛋白酶（TRP）降低（p <.05）。饮食LCSM替代上调了肠肿瘤坏死因子-α（TNF-α），激活的B细胞核因子κ轻链增强子（NF-κB）和白介素1 beta（IL-1β）的表达，但是下调紧密连接蛋白ZO-1（ZO-1），转化生长因子β-3（TGF-β3）和白介素10（IL-10）的表达。组织学分析显示，随着FM置换水平的提高，肠中段逐渐受到形态学损害。这些结果表明，与基于FM的对照相比，被LCSM补充氨基酸（蛋氨酸和赖氨酸）的88.5 g / kg（16％）FM不会显着降低生长。