The red claw (RC) crayfish (Cherax quadricarinatus) is one of the most important farmed freshwater crustacean species for its high palatability, market value and consumer preference. Understanding the role of diets in maturation can help to overcome some of the challenges to reproduction. Optimal effect of dietary vitamin E (VE) supplementation, Arachidonic acid (ARA), Haemtococcus pluvialis (H.P) nucleotide (NT) and yeast extract (YE) on the growth and ovarian development of RC crayfish is determined in the present study. The range analysis of a L16 (4⁵) orthogonal array design arranged five factors varied at four levels (VE of 40, 160, 240 and 320 mg/kg; NT of 2, 4, 6 and 8 g/kg; H.P of 2, 4, 6 and 8 g/kg; ARA of 5, 10, 15 and 20 g/kg; and YE of 2.5, 5, 10 and 15 g/kg) revealed that the highest weight gain (WG), feed conversion ratio (FCR) and gonadosomatic index (GSI) of the crayfish occurred at VE of 240 mg/kg, NT of 8 g/kg, H.P 4 g/kg, ARA of 5 g/kg and YE of 10 g/kg, and the maximum hepatosomatic index was observed at VE of 40 mg/kg, NT of 4 g/kg, H.P of 4 g/kg, ARA of 10 g/kg and YE of 5 g/kg. Furthermore, analysis of variance demonstrated that all the five factors had significant effects on WG, GSI and FCR. In contrast, any of these factors did not have a significant influence on the hepatosomatic index and SR. These results proposed that dietary contains VE of 240 mg/kg, NT of 8 g/kg, H.P of 4 g/kg, ARA of 5 g/kg and YE of 10 g/kg might be the optimal combinations for the growth and ovarian development of RC crayfish.

中文翻译：

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正交试验设计维生素E，花生四烯酸，雨生红球菌，核苷酸和酵母提取物对小龙虾（Cherax quadricarinatus）生长和卵巢发育的综合影响

红爪（RC）小龙虾（Cherax quadricarinatus）因其高适口性，市场价值和消费者喜好而成为最重要的淡水甲壳类养殖品种之一。了解饮食在成熟中的作用可以帮助克服生殖方面的一些挑战。本研究确定了膳食维生素E（VE），花生四烯酸（ARA），雨生红球菌（HP）核苷酸（NT）和酵母提取物（YE）对RC小龙虾生长和卵巢发育的最佳作用。L16（4 ⁵）正交阵列设计将五个因子排列成四个水平（VE为40、160、240和320 mg / kg; NT为2、4、6和8 g / kg; HP为2、4、6和8 g / kg ； ARA分别为5、10、15和20 g / kg； YE为2.5、5、10和15 g / kg）显示出最高的增重（WG），饲料转化率（FCR）和性腺激素指数（GSI）在VE为240 mg / kg，NT为8 g / kg，HP为4 g / kg，ARA为5 g / kg和YE为10 g / kg时出现了小龙虾，并且在VE为40时观察到最大的肝体指数mg / kg，NT为4 g / kg，HP为4 g / kg，ARA为10 g / kg和YE为5 g / kg。此外，方差分析表明，所有五个因素均对WG，GSI和FCR产生了显着影响。相比之下，这些因素中的任何一个对肝体指数和SR均没有显着影响。这些结果表明，饮食中的VE含量为240 mg / kg，NT为8 g / kg，H。