There is considerable concern that naphthenic acids (NA) related to oil extraction can negatively impact reproduction in mammals, yet the mechanisms are unknown. Since placental dysfunction is central to many adverse pregnancy outcomes, the goal of this study was to determine the effects of NA exposure on placental trophoblast cell function. HTR-8/SVneo cells were exposed to a commercial technical NA mixture for 24 hours to assess transcriptional regulation of placentation-related pathways and functional assessment of migration, invasion, and angiogenesis. Pathway analysis suggests that NA treatment resulted in increased epithelial-to-mesenchymal transition. However, there was reduced migration and invasive potential. NA treatment increased angiogenesis-related pathways with a concomitant increase in tube formation. Since decreased trophoblast invasion/migration and aberrant angiogenesis have been associated with placental dysfunction, these findings suggest that it is biologically plausible that exposure to NA may result in altered placental development and/or function.

人们相当担心与石油提取相关的环烷酸 (NA) 会对哺乳动物的繁殖产生负面影响，但其机制尚不清楚。由于胎盘功能障碍是许多不良妊娠结局的核心，本研究的目的是确定 NA 暴露对胎盘滋养层细胞功能的影响。HTR-8/SVneo 细胞暴露于商业技术 NA 混合物 24 小时，以评估胎盘相关通路的转录调控和迁移、侵袭和血管生成的功能评估。通路分析表明，NA 治疗导致上皮间质转化增加。然而，迁移和侵入潜力减少。NA 治疗增加了血管生成相关通路，同时管形成增加。