Abstract Probioceuticals are probiotic-derived biologically active compounds that positively influence human health. Exopolysaccharides (EPS) are long chain polymers of sugars and their derivatives with diverse biological functions. The objective of this work was to study EPS from Pediococcus acidilactici (PA) NCDC 252, a potential probiotic. In silico analysis of PA NCDC 252 genome revealed an EPS gene cluster (10 genes) and genes were further analysed for their functional domains and products. EPS from PA NCDC 252 were purified by ethanol precipitation and gel filtration chromatography on Sephadex G-100. Molecular mass of purified EPS was 89.1 KDa as determined by gel filtration chromatography. Structural and component analysis by FTIR, NMR and UPLC revealed purified EPS to be linear homopolysaccharides (α-glucans) with few α-(1→3) branches. in vitro studies showed anti-oxidant, reduction potential and anti-cancer activity in dose dependent manner. Total antioxidant potential of EPS was 11.9 %. In-vitro cell viability assay revealed anti-proliferative effect of EPS on human colon cancer cell line (HCT116). At 10 and 100 μg/mL, EPS inhibited HCT116 cells up to 67.1 % and 87.3 % respectively. These results suggest that PA EPS can be therapeutically used as anti-oxidant and anticancer agent after in vivo studies.

中文翻译：

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来自潜在益生菌乳酸片球菌 NCDC 252 的胞外多糖的纯化、部分结构表征和健康益处

摘要 Probioceuticals 是益生菌衍生的生物活性化合物，对人类健康有积极影响。胞外多糖（EPS）是糖类及其衍生物的长链聚合物，具有多种生物学功能。这项工作的目的是研究来自乳酸片球菌 (PA) NCDC 252（一种潜在的益生菌）的 EPS。PA NCDC 252 基因组的计算机分析揭示了一个 EPS 基因簇（10 个基因），并进一步分析了基因的功能域和产物。来自 PA NCDC 252 的 EPS 通过乙醇沉淀和凝胶过滤层析在 Sephadex G-100 上纯化。经凝胶过滤色谱法测定，纯化的 EPS 的分子量为 89.1 KDa。FTIR、NMR 和 UPLC 的结构和成分分析表明，纯化的 EPS 是线性同多糖（α-葡聚糖），具有很少的 α-(1→3) 分支。体外研究显示其具有剂量依赖性的抗氧化、还原电位和抗癌活性。EPS 的总抗氧化潜力为 11.9%。体外细胞活力测定揭示了 EPS 对人结肠癌细胞系 (HCT116) 的抗增殖作用。在 10 和 100 μg/mL 时，EPS 对 HCT116 细胞的抑制率分别高达 67.1% 和 87.3%。这些结果表明，在体内研究后，PA EPS 可以在治疗上用作抗氧化剂和抗癌剂。