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A clearing protocol for Galleria mellonella larvae: Visualization of internalized fluorescent nanoparticles
New Biotechnology ( IF 4.5 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.nbt.2020.08.002
Laura Moya-Andérico 1 , Joana Admella 1 , Eduard Torrents 2
Affiliation  

Light scattering is a challenge for imaging three-dimensional organisms. A number of new tissue clearing methodologies have been described in recent years, increasing the utilities of clearing techniques to obtain transparent samples. Here, we describe the optimization of a suitable and novel protocol for clearing Galleria mellonella larvae, an alternative infection animal model with a promising potential for the toxicological evaluation of different molecules and materials. This has allowed the visualization of internalised fluorescent nanoparticles using confocal microscopy, opening the door to a wide range of different applications.

中文翻译:

大蜡螟幼虫的清除方案:内化荧光纳米粒子的可视化

光散射是三维生物成像的一个挑战。近年来已经描述了许多新的组织清除方法,增加了清除技术获得透明样本的效用。在这里,我们描述了用于清除大蜡螟幼虫的合适和新颖的协议的优化,这是一种替代感染动物模型,具有对不同分子和材料进行毒理学评估的潜力。这使得使用共聚焦显微镜可视化内化荧光纳米粒子,为广泛的不同应用打开了大门。
更新日期:2021-01-01
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