Lignocellulosic biomass is an abundant and renewable feedstock for biofuels, biochemicals and biomaterials. To make this recalcitrant biomass more susceptible to hydrolysis and microbial conversion a pretreatment is required. Many pretreatments have resulted in high sugar yields; however, they can generate microbial inhibitory compounds in the fermentation step. Therefore, the detoxification of hydrolysates is necessary. This study shows that reduced graphene oxide adorned with magnetite nanoparticles (rGO-MNPs) can adsorb inhibitory compounds such as furfural. Adsorption tests were performed in both aqueous solutions (furfural and furfural-glucose) and wood hydrolysate. rGO-MNPs (20 mg) removed 68% of furfural when it was alone, 49% of furfural when it was mixed with glucose and 20% of furfural in the wood hydrolysate. Among the adsorption of furfural in wood hydrolysate, rGO-MNPs adsorbed other lignin derivate compounds. Finally, no toxic effect of rGO-MNPs on R. toruloides-1588 was observed during the fermentation using detoxified wood hydrolysate.

木质纤维素生物质是一种丰富的可再生原料，用于生物燃料，生物化学物质和生物材料。为了使这种顽固的生物质更易于水解和微生物转化，需要进行预处理。许多预处理导致了高糖产量。但是，它们可以在发酵步骤中产生微生物抑制性化合物。因此，必须对水解产物进行解毒。这项研究表明，装饰有磁铁矿纳米颗粒（rGO-MNPs）的还原氧化石墨烯可以吸附抑制性化合物，例如糠醛。在水溶液（糠醛和糠醛葡萄糖）和木材水解物中均进行了吸附测试。rGO-MNP（20 mg）单独使用时，可去除68％的糠醛，与葡萄糖混合时可去除49％的糠醛，在木材水解物中可去除20％的糠醛。在木材水解产物中糠醛的吸附中，rGO-MNPs吸附了其他木质素衍生化合物。最后，rGO-MNPs对使用解毒的木材水解物在发酵过程中观察到了R. toruloides -1588。