The snail Bellamya purificata is an ecologically and economically important freshwater gastropod species. However, limited genomic resources are available for this snail. In this study, the transcriptome of mantle tissues and proteome of shells of B. purificata with two shell colors (namely light-cyan line (LC) and light-purple line (LP)) were deeply sequenced and characterized. A total of 5.72 million contigs were assembled into 157,015 unigenes, 21,455 (13.66%) of these unigenes were significantly matched to NR, Swiss-Prot, KOG, GO and KEGG database. 1807 differentially expressed genes (DEGs) were identified between the two different shell color lines. These DEGs were significantly enriched in five KEGG pathways including tyrosine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, and histidine metabolism, which suggested that the shell color polymorphism in B. purificata was a result of melanin synthesis variation. A total of 1521 proteins were identified in B. purificata here as well. The differentially expressed protein analysis showed that the tyrosinase content in LP was significantly decreased in comparison to LC, which agreed with the transcriptome analysis results. This study provides valuable genomic resources of B. purificata and improves our understanding of molecular mechanisms of biomineralization and shell color polymorphism in snail.

蜗牛Bellamya purificata是一种具有生态和经济意义的淡水腹足动物。但是，有限的基因组资源可用于此蜗牛。在这项研究中，地幔组织的转录组和蛋白质组的壳B. purificata对具有两种外壳颜色（即浅青色线（LC）和浅紫色线（LP））的分子进行了深度测序和表征。总计572万个重叠群被整合为157,015个单基因，其中21,455个（13.66％）与NR，Swiss-Prot，KOG，GO和KEGG数据库显着匹配。在两个不同的壳色线之间鉴定出1807个差异表达基因（DEG）。这些DEGS五个KEGG途径，包括酪氨酸代谢，色氨酸代谢，苯丙氨酸，酪氨酸和色氨酸生物合成，苯丙氨酸代谢，和组氨酸代谢，表明所述壳的颜色多态性均显著富集B. purificata是黑色素合成变化的结果。在B. purificata中共鉴定出1521种蛋白质这里也是。差异表达的蛋白质分析表明，与LC相比，LP中酪氨酸酶的含量明显降低，这与转录组分析结果相符。这项研究提供了宝贵的B. purificata基因组资源，并增进了我们对蜗牛的生物矿化和壳颜色多态性的分子机制的理解。