Soybean isoflavone aglycones have been investigated as potential wound healing compounds for topical application. The aim of this study was to evaluate the wound healing properties of a soybean isoflavone aglycones-rich fraction (IAF) when incorporated into lipid nanoemulsions dispersed in acrylic-acid hydrogels. Formulations exhibited a mean droplet size in the sub 200 nm range, negative ζ-potential (−60 mV), and displayed non-Newtonian pseudoplastic behavior. The addition of a gelling agent decreased the IAF release from formulations and improved the retention of these compounds in intact porcine ear skin when compared with a control propylene glycol solution. No IAF were detected in receptor fluid of Franz-type diffusion cells. However, increasing amounts of IAF were noticed in both skin layers and the receptor fluid when the tissue was partially injured (tape stripping), or when the epidermis was completely removed. In vitro studies showed that IAF elicits an increased proliferation and migration of keratinocytes (HaCaT cell line). Subsequently, the healing effect of the formulations was evaluated in a model of dorsal wounds in rats, by assessing the size of the lesions, histology, inflammatory markers, and antioxidant activity. Overall findings demonstrated the potential of IAF-loaded formulations to promote wound healing by increasing angiogenesis by ∼200 %, reducing the lipid oxidation (TBARS) by ∼52 % and the inflammation (TNFα) by ∼35 %, while increasing re-epithelialization by ∼500 %, visualized by the epithelium thickness.

大豆异黄酮苷元已被研究作为局部应用的潜在伤口愈合化合物。本研究的目的是评估富含大豆异黄酮苷元的组分 (IAF) 掺入分散在丙烯酸水凝胶中的脂质纳米乳液中时的伤口愈合特性。制剂的平均液滴尺寸在 200 nm 以下，负 ze 电势 (−60 mV)，并表现出非牛顿假塑性行为。与对照丙二醇溶液相比，胶凝剂的添加减少了 IAF 从制剂中的释放，并改善了这些化合物在完整猪耳皮肤中的保留。 Franz型扩散池的受体液中未检测到IAF。然而，当组织部分损伤（胶带剥离）或表皮完全去除时，皮肤层和受体液中的 IAF 量都会增加。体外研究表明，IAF 会引起角质形成细胞（HaCaT 细胞系）的增殖和迁移增加。随后，通过评估病变大小、组织学、炎症标志物和抗氧化活性，在大鼠背部伤口模型中评估了制剂的愈合效果。总体研究结果表明，负载 IAF 的制剂具有促进伤口愈合的潜力，可将血管生成增加约 200%，将脂质氧化 (TBARS) 减少约 52%，将炎症 (TNFα) 减少约 35%，同时将上皮再形成增加约 10%。 ∼500%，通过上皮厚度可视化。