Abstract

Bifidobacteria are considered key commensals of the human intestinal tract; they dominate the community in the earliest stages of life and are the first to respond to stress factors. The metasequencing of the V3–V4 16S rRNA fragments made it possible to identify 12 phylotypes of bifidobacteria in the gut microbiome of adolescents, which accounted for from 0.0001 to 0.9% of the total microbiome. A phylogenetic analysis of the obtained phylotypes was conducted; such species as B. angulatum, B. bifidum, B. longum, and B. animalis, as well as subspecies B. animalis subsp. lactis, were identified. The species of bifidobacteria excluded from identification by the V3–V4 variable regions were identified. The phylogenetic analysis of different variable regions and their combinations showed that a tree topology based on the V2 fragment is the most similar to a tree topology based on the complete gene.

中文翻译：

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基于16S rRNA基因可变区的双歧杆菌分类学鉴定能力

摘要

双歧杆菌被认为是人类肠道的重要标志。他们在生命的最初阶段统治社区，并且是第一个对压力因素做出反应的人。V3-V4 16S rRNA片段的元测序使我们有可能鉴定出青少年肠道微生物组中双歧杆菌的12种系统型，占总微生物组的0.0001％至0.9％。对获得的系统型进行了系统发育分析。例如B. angulatum，B. bifidum，B. longum和B. animalis以及亚种B. animalis亚种。乳酸菌，已被识别。鉴定了被V3-V4可变区排除在鉴定之外的双歧杆菌种类。对不同可变区及其组合的系统进化分析表明，基于V2片段的树形拓扑与基于完整基因的树形拓扑最相似。