The aim of the study is to develop transgenic rice with annexin genes (AnnBj2) to determine its salinity and water stress tolerance through Agrobacterium-mediated genetic transformation. The transgenic somatic embryos were developed on Murashige and Skoog medium fortified with 0.5 mg L⁻¹ kinetin and 3.0 mg L⁻¹ 2,4-D. About 80% of the transformed somatic embryos germinated and was successfully established in the transgenic greenhouse. The transformed plantlets (T₀ and T₁ generation) were identified through PCR using NPT-II and AnnBj2 gene–specific primers as well as positive uidA reporter gene expression. Compared with non-transformed plants, the transgenic rice plants overexpressing AnnBj2 gene exhibited salt tolerance at the seedling stage. Seeds generated at T₀ and T₁ generations were further studied to determine their salinity tolerance through nutrient culture and pot culture experiments for water stress. When compared with the non-transgenic control, transgenic seeds (T₀ and T₁) had increased germination on a nutrient medium having 200 mM NaCl. The data showed that the shoot and root lengths were longer at 200 mM NaCl when compared with those of the non-transformed plants. Further, the biochemical assessment (photosynthetic pigment analysis, protein, proline content, and oxidative enzyme activity) was performed between transformed (T₀ and T₁) and non-transformed plantlets under stress. It was noted that the transformed plantlets were higher in chlorophyll and proline content as well as oxidative enzyme activity as compared with the non-transformed plants.

该研究的目的是开发具有膜联蛋白基因（AnnBj2）的转基因水稻，以通过农杆菌介导的遗传转化来确定其盐度和水分胁迫耐受性。转基因体细胞胚在强化有0.5 mg L ^-1激动素和3.0 mg L ^-1 2,4-D的Murashige和Skoog培养基上发育。约80％的转化体细胞胚已发芽，并成功地在转基因温室中建立。使用NPT-II和AnnBj2基因特异性引物以及uidA阳性通过PCR鉴定了转化的幼苗（T ₀和T ₁代）报告基因的表达。与未转化植物相比，过表达AnnBj2基因的转基因水稻植物在苗期表现出耐盐性。通过营养培养和盆栽试验针对水分胁迫，进一步研究了在T ₀和T ₁世代产生的种子的耐盐性。与非转基因对照相比，转基因种子（T ₀和T ₁）在含有200 mM NaCl的营养培养基上发芽增加。数据显示，与未转化植物相比，在200 mM NaCl处的芽和根长更长。此外，在胁迫下在转化的（T ₀和T ₁）和未转化的幼苗之间进行了生化评估（光合色素分析，蛋白质，脯氨酸含量和氧化酶活性）。注意到，与未转化的植物相比，转化的植物的叶绿素和脯氨酸含量以及氧化酶活性更高。