In this study, the anti-oxidative and neuro-protective effects of ethanolic extracts of the dried roots of Allium hookeri were investigated. Total phenolic contents and total flavonoid contents of A. hookeri extract depended on the ethanol concentrations used (50, 70 and 95%). In order to evaluate radical scavenging activity, DPPH and ABTS radical scavenging assays and ferric reducing powers were evaluated. The results showed the 95% ethanol extract of A. hookeri (95AH) had higher phenolic and flavonoid contents, and greater radical scavenging activities than 50 or 70% ethanol extracts of A. hookeri. The neuro-protective effects of 95AH were evaluated using H₂O₂-treated PC12 neuronal cells. Treatment of 95AH increased cell viability and superoxide dismutase and glutathione peroxidase activities, reduced lactate dehydrogenate release, reduced reactive oxygen species production, and increased Bcl-2/Bax ratio. HPLC revealed 95AH was rich in phenolics, especially catechin. These results demonstrate 95AH has substantial anti-oxidative and neuro-protective effects against H₂O₂-induced oxidative stress.

在这项研究中，抗氧化和的干燥根的乙醇提取物的神经保护作用宽叶进行了调查。A. hookeri提取物的总酚含量和总黄酮含量取决于所使用的乙醇浓度（50%、70% 和 95%）。为了评估自由基清除活性，评估了 DPPH 和 ABTS 自由基清除试验和三价铁还原能力。结果表明的95％乙醇提取物A.木莲（95AH）具有更高的酚和类黄酮含量，和比为50种或70％乙醇提取物更高的自由基清除活性A.木莲。使用 H ₂ O ₂评估 95AH 的神经保护作用-处理的 PC12 神经元细胞。95AH 的处理增加了细胞活力和超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，减少了乳酸脱氢释放，减少了活性氧的产生，并增加了 Bcl-2/Bax 比率。HPLC 显示 95AH 富含酚类物质，尤其是儿茶素。这些结果表明，95AH 对 H ₂ O ₂诱导的氧化应激具有显着的抗氧化和神经保护作用。