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Contribution of male genitors in open-pollination progenies of sour passion fruit
Euphytica ( IF 1.6 ) Pub Date : 2020-08-29 , DOI: 10.1007/s10681-020-02681-z
Maria Helena Menezes Cordeiro , Claudio Horst Bruckner , Renato Domiciano Silva Rosado , Carlos Eduardo Magalhães dos Santos , Eveline Teixeira Caixeta , Tiago Vieira Sousa , Cosme Damião Cruz

Passion fruit breeding programs often use a single fruit to obtain progeny. Open-pollination progenies are considered half-siblings since they allow the occurrence of a mixture of pollens during pollination. However, there are no studies able to prove that these progenies are, in fact, consequence of a mixture of pollens. The contribution of male genitors to the formation of open-pollinated progenies and genetic diversity were evaluated by means of microsatellite markers. A hybrid test was performed based on the gathered genotypic data, a chi-square test was used to verify the expected and observed frequencies, and the proportion of identical genes by descent (IBD) was estimated. Genetic diversity was studied through descriptive analysis, cluster analysis, and molecular analysis (AMOVA). The hybrid test indicated the participation of more than one male genitor in progeny formation. However, the genotypic composition observed in the progenies differed significantly from that expected when assuming crosses between all individuals of the origin population. The IBD values for the progenies ranged from 0.26 to 0.30, indicating that they are progenies of half-siblings. The progenies showed observed heterozygosity greater than expected and an inbreeding coefficient close to zero. The AMOVA revealed that the greatest genetic variation (81.96%) occurred within progenies. In sour passion fruit, open-pollinated progenies obtained from a single fruit do not present genotypic composition representative of their population of origin since the greatest genetic diversity was observed within the progenies.

中文翻译:

雄性生殖器对酸百香果开放授粉后代的贡献

百香果育种计划通常使用单个果实来获得后代。开放授粉后代被认为是半同胞,因为它们允许在授粉过程中出现混合花粉。然而,没有研究能够证明这些后代实际上是花粉混合的结果。通过微卫星标记评估雄性生殖器对开放授粉后代形成和遗传多样性的贡献。基于收集到的基因型数据进行混合测试,使用卡方检验来验证预期和观察到的频率,并估算出相同基因的血统比例 (IBD)。通过描述性分析、聚类分析和分子分析 (AMOVA) 研究遗传多样性。杂交试验表明不止一个雄性生殖器参与了后代的形成。然而,在后代中观察到的基因型组成与假设原始种群所有个体之间的杂交时的预期显着不同。后代的 IBD 值范围从 0.26 到 0.30,表明它们是半同胞的后代。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。然而,在后代中观察到的基因型组成与假设原始种群所有个体之间的杂交时的预期显着不同。后代的 IBD 值范围从 0.26 到 0.30,表明它们是半同胞的后代。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。然而,在后代中观察到的基因型组成与假设原始种群所有个体之间的杂交时的预期显着不同。后代的 IBD 值范围从 0.26 到 0.30,表明它们是半同胞的后代。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。在后代中观察到的基因型组成与假设原始种群所有个体之间的杂交时的预期显着不同。后代的 IBD 值范围从 0.26 到 0.30,表明它们是半同胞的后代。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。在后代中观察到的基因型组成与假设原始种群所有个体之间的杂交时的预期显着不同。后代的 IBD 值范围从 0.26 到 0.30,表明它们是半同胞的后代。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。后代显示观察到的杂合度大于预期,近交系数接近于零。AMOVA 显示最大的遗传变异 (81.96%) 发生在后代中。在酸百香果中,从单个果实中获得的开放授粉后代不存在代表其起源种群的基因型组成,因为在后代中观察到最大的遗传多样性。
更新日期:2020-08-29
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