Circular RNAs (circRNAs) may be potential biomarkers or therapeutic targets of hemophagocytic syndrome（HPS）due to their high stability, covalently closed structure and implicated roles in gene regulation. The aim of this study was to determine and characterize the circRNAs from NK/T-cell lymphoma-associated hemophagocytic syndrome（NK/T-LAHS）. CircRNA in NK/T-LAHS and healthy control patient serum were assessed using next-generation sequencing. 143 differentially expressed circRNAs of which 114 were up-regulated and 29 were down-regulated in NK/T-LAHS patients were identified. Next, GO (Gene Ontology) function and KEGG (Kyoto encyclopedia of genes and genomes) pathway analyses to explore the roles of these circRNAs were utilized, and a miRNA target gene prediction software to predict the interaction of circRNAs and miRNAs was used. Moreover, 5 circRNAs were then selected as NK/T-LAHS candidate circRNAs which were related to tumors and contained NK/T-LAHS-related miRNA binding sites. Using real-time PCR, the significant upregulation of these 5 circRNAs in NK/T-LAHS patient serum were verified. Together these results show that circRNAs may serve as valuable diagnostic biomarkers of early NK/T-LAHS, with potential therapeutic targets in disease progression.

中文翻译：

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分析和生物信息学分析显示在NK / T细胞淋巴瘤相关的噬血细胞综合征中差异环状RNA表达。

环状RNA（circRNA）由于其高稳定性，共价闭合结构以及在基因调控中的作用，可能是潜在的噬血细胞综合征（HPS）的生物标志物或治疗靶标。本研究的目的是确定和鉴定NK / T细胞淋巴瘤相关的噬血细胞综合征（NK / T-LAHS）的circRNA。使用下一代测序评估NK / T-LAHS和健康对照患者血清中的CircRNA。在NK / T-LAHS患者中，鉴定了143个差异表达的circRNA，其中114个被上调，而29个被下调。接下来，利用GO（基因本体论）功能和KEGG（基因和基因组京都百科全书）途径分析来探索这些circRNA的作用，并使用miRNA目标基因预测软件来预测circRNA和miRNA的相互作用。此外，然后选择5个circRNA作为与肿瘤相关的NK / T-LAHS候选circRNA，并包含与NK / T-LAHS相关的miRNA结合位点。使用实时PCR，验证了NK / T-LAHS患者血清中这5个circRNA的显着上调。这些结果共同表明，circRNAs可以作为早期NK / T-LAHS的有价值的诊断生物标志物，在疾病进展中具有潜在的治疗靶点。