Pteris cretica (L.) commonly known as Cretan brake is widely distributed in nature and considered as potential environmental carcinogen. However, only limited literature is available on this fern commonly taken by cattle while grazing. It is known that in cattle Bovine papilloma virus (BPV) induced benign tumours are converted into malignant with interaction of ptaquiloside (Pta) present in ferns. In the present investigation, we evaluated the pathological effects of P. cretica (PC)-BPV infection in laboratory model hamster. Though toxic principle Pta was detected in fern samples but quercetin could not be found. Tumours were successfully induced in hamsters by cutaneous wart suspension and histopathologically diagnosed as fibroma and lipofibroma. Histopathologically, hamsters showed mild to moderate vascular changes in vital organs, multiple cysts, degenerative changes, bile duct hyperplasia and necrosis in liver, haemorrhages and haemosiderosis in spleen, hypersecretory activity and prominent Peyer’s patches in ileum, degenerative changes and presence of eosinophilic casts in renal tubules. Ultrastructural study revealed apoptosis in hepatocytes, abundance of variable shaped mitochondria in renal tubular lining epithelial cells and enterocytes showed abundance of mitochondria and cytoplasmolysis in the fern fed groups. Almost all the hamsters from BPV, fern and virus infection (PC+BPV) groups developed similar type of tumorous growths. The visible growths in the hamsters of these groups were either single or double large sized except multiple tumorous growths in one hamster from PC+BPV group. However, multiple palpable subcutaneous nodules were developed at the site of scarification in all the hamsters of BPV and PC+BPV groups. Our findings suggest that the Pta containing P. cretica feeding induced hepatotoxic and nephrotoxic lesions in hamsters, but effects of P. cretica-BPV infection were found negligible.

中文翻译：

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叙利亚黄金仓鼠（Mesocricetus auratus）中的凤尾蕨蕨牛牛乳头瘤病毒感染的病理研究

Pteris cretica（L.）俗称Cretan刹车，在自然界广泛分布，被认为是潜在的环境致癌物。然而，关于牛通常在放牧时所采摘的这种蕨类植物的文献很少。众所周知，在牛中，牛乳头瘤病毒（BPV）诱导的良性肿瘤会与蕨类植物中的Ptaquiloside（Pta）相互作用而转化为恶性肿瘤。在本研究中，我们评估了景天体育的病理影响（PC）-BPV感染在实验室模型仓鼠中。尽管在蕨类样品中检测到了毒性原理Pta，但未发现槲皮素。皮肤疣悬液成功地在仓鼠中诱导了肿瘤，并且在组织病理学上被诊断为纤维瘤和脂纤维瘤。从组织病理学上看，仓鼠在重要器官中显示出轻至中度的血管变化，多个囊肿，变性，肝脏胆管增生和坏死，脾脏出血和铁血黄素沉着，分泌过多活动和回肠明显的Peyer's斑，变性和嗜酸性粒细胞的存在肾小管。超微结构研究显示，在蕨类动物喂养的组中，肝细胞凋亡，肾小管衬里上皮细胞中大量异型线粒体和肠上皮细胞显示了线粒体丰度和细胞质溶解。BPV，蕨类和病毒感染（PC + BPV）组的几乎所有仓鼠都出现了相似类型的肿瘤生长。这些组的仓鼠中可见的生长为单个或两倍大，除了来自PC + BPV组的一只仓鼠中的多个肿瘤生长。然而，在BPV和PC + BPV组的所有仓鼠中，在划痕部位均出现了多个明显的皮下结节。我们的发现表明，含有 在BPV和PC + BPV组的所有仓鼠中，在划痕部位均出现了多个明显的皮下结节。我们的发现表明，含有 在BPV和PC + BPV组的所有仓鼠中，在划痕部位均出现了多个明显的皮下结节。我们的发现表明，含有食用P. cretica引起仓鼠的肝毒性和肾毒性损害，但发现P. cretica -BPV感染的影响可忽略不计。