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Irreversible and reversible morphological changes in the φ6 capsid and similar viral shells: symmetry and micromechanics.
Soft Matter ( IF 2.9 ) Pub Date : 2020-08-27 , DOI: 10.1039/d0sm01338b Olga V Konevtsova 1 , Daria S Roshal 1 , Rudolf Podgornik 2 , Sergei B Rochal 1
Soft Matter ( IF 2.9 ) Pub Date : 2020-08-27 , DOI: 10.1039/d0sm01338b Olga V Konevtsova 1 , Daria S Roshal 1 , Rudolf Podgornik 2 , Sergei B Rochal 1
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Understanding the physicochemical processes occurring in viruses during their maturation is of fundamental importance since only mature viruses can infect host cells. Here we consider the irreversible and reversible morphological changes that occur with the dodecahedral ϕ6 procapsid during the sequential packaging of 3 RNA segments forming the viral genome. It is shown that the dodecahedral shape of all the four observed capsid states is perfectly reproduced by a sphere radially deformed by only two irreducible spherical harmonics with icosahedral symmetry and wave numbers l = 6 and l = 10. The rotation of proteins around the 3-fold axes at the Procapsid → Intermediate 1 irreversible transformation is in fact also well described with the shear field containing only two irreducible harmonics with the same two wave numbers. The high stability of the Intermediate 1 state is discussed and the shapes of the Intermediate 2 state and Capsid (reversibly transforming back to the Intermediate 1 state) are shown to be mainly due to the isotropic pressure that the encapsidated RNA segments exert on the shell walls. The hidden symmetry of the capsid and the physicochemical features of the in vitro genome extraction from the viral shell are also elucidated.
中文翻译：
φ6衣壳和类似病毒壳中不可逆和可逆的形态变化：对称性和微力学。
了解病毒在成熟过程中发生的物理化学过程至关重要，因为只有成熟的病毒才能感染宿主细胞。在这里，我们考虑了十二面体pro 6衣壳在顺序包装形成病毒基因组的3个RNA片段时发生的不可逆和可逆形态变化。结果表明，所观察到的所有四个衣壳状态的十二面体形状都可以被球体完美地再现，该球体仅通过两个具有二十面体对称性且波数分别为l = 6和l的不可约球谐函数径向变形= 10.蛋白质在Procapsid→中间1不可逆转换时围绕3倍轴的旋转实际上也很好地描述了，其剪切场仅包含两个具有相同两个波数的不可约谐波。讨论了中间体1状态的高稳定性，中间体2状态和衣壳的形状（可逆地转换回中间体1状态）主要是由于衣壳化RNA片段施加在壳壁上的各向同性压力。还阐明了衣壳的隐藏对称性和从病毒壳中提取体外基因组的理化特征。
更新日期：2020-09-20
中文翻译：
φ6衣壳和类似病毒壳中不可逆和可逆的形态变化：对称性和微力学。
了解病毒在成熟过程中发生的物理化学过程至关重要，因为只有成熟的病毒才能感染宿主细胞。在这里，我们考虑了十二面体pro 6衣壳在顺序包装形成病毒基因组的3个RNA片段时发生的不可逆和可逆形态变化。结果表明，所观察到的所有四个衣壳状态的十二面体形状都可以被球体完美地再现，该球体仅通过两个具有二十面体对称性且波数分别为l = 6和l的不可约球谐函数径向变形= 10.蛋白质在Procapsid→中间1不可逆转换时围绕3倍轴的旋转实际上也很好地描述了，其剪切场仅包含两个具有相同两个波数的不可约谐波。讨论了中间体1状态的高稳定性，中间体2状态和衣壳的形状（可逆地转换回中间体1状态）主要是由于衣壳化RNA片段施加在壳壁上的各向同性压力。还阐明了衣壳的隐藏对称性和从病毒壳中提取体外基因组的理化特征。
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