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The biomolecule corona of lipid nanoparticles contains circulating cell-free DNA.
Nanoscale Horizons ( IF 8.0 ) Pub Date : 2020-08-27 , DOI: 10.1039/d0nh00333f
Lois Gardner 1 , Jessica Warrington , Jane Rogan , Dominic G Rothwell , Ged Brady , Caroline Dive , Kostas Kostarelos , Marilena Hadjidemetriou
Affiliation  

The spontaneous adsorption of biomolecules onto the surface of nanoparticles (NPs) in complex physiological biofluids has been widely investigated over the last decade. Characterisation of the protein composition of the ‘biomolecule corona’ has dominated research efforts, whereas other classes of biomolecules, such as nucleic acids, have received no interest. Scarce, speculative statements exist in the literature about the presence of nucleic acids in the biomolecule corona, with no previous studies attempting to describe the contribution of genomic content to the blood-derived NP corona. Herein, we provide the first experimental evidence of the interaction of circulating cell-free DNA (cfDNA) with lipid-based NPs upon their incubation with human plasma samples, obtained from healthy volunteers and ovarian carcinoma patients. Our results also demonstrate an increased amount of detectable cfDNA in patients with cancer. Proteomic analysis of the same biomolecule coronas revealed the presence of histone proteins, suggesting an indirect, nucleosome-mediated NP–cfDNA interaction. The finding of cfDNA as part of the NP corona, offers a previously unreported new scope regarding the chemical composition of the ‘biomolecule corona’ and opens up new possibilities for the potential exploitation of the biomolecule corona for the enrichment and analysis of blood-circulating nucleic acids.

中文翻译:


脂质纳米颗粒的生物分子冠含有循环游离DNA。



在过去的十年中,生物分子自发吸附到复杂生理生物流体中的纳米粒子(NP)表面上已得到广泛研究。 “生物分子冠”蛋白质组成的表征主导了研究工作,而其他类别的生物分子,例如核酸,却没有引起人们的兴趣。文献中关于生物分子冠中核酸存在的推测性陈述很少,之前没有研究试图描述基因组内容对血液来源的 NP 冠的贡献。在此,我们提供了第一个实验证据,证明循环游离 DNA (cfDNA) 与脂质纳米粒子在与从健康志愿者和卵巢癌患者获得的人血浆样本一起孵育后相互作用。我们的结果还表明,癌症患者中可检测到的 cfDNA 数量有所增加。对相同生物分子冠的蛋白质组学分析揭示了组蛋白的存在,表明核小体介导的间接 NP-cfDNA 相互作用。 cfDNA 作为 NP 冠的一部分的发现,为“生物分子冠”的化学成分提供了先前未报道的新范围,并为利用生物分子冠来富集和分析血液循环核酸开辟了新的可能性酸。
更新日期:2020-08-28
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