We present a base editing system, in which base editors are attached to different sites of sgRNA scaffold (sgBE). Each independent sgBE has its own specific editing pattern for a given target site. Among tested sgBEs, sgBE-SL4, in which deaminase is attached to the last stem-loop of sgRNA, yields the highest editing efficiency in the window several nucleotides next to the one edited by BE3. sgBE enables the simultaneous editing of adenine and cytosine. Finally, in order to facilitate in vivo base editing, we extend our sgBE system to an AAV-compatible Cas9, SaCas9 ( Staphylococcus aureus ), and observe robust base editing.

中文翻译：

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sgBE：sgRNA架构的结构引导设计指定碱基编辑窗口并实现胞嘧啶和腺苷的同时转换


我们提出了一个碱基编辑系统，其中碱基编辑器附加到 sgRNA 支架（sgBE）的不同位点。每个独立的 sgBE 对于给定的目标位点都有其自己特定的编辑模式。在测试的 sgBE 中，sgBE-SL4（其中脱氨酶附着在 sgRNA 的最后一个茎环上）在窗口中的几个核苷酸中产生最高的编辑效率，仅次于 BE3 编辑的核苷酸。 sgBE 能够同时编辑腺嘌呤和胞嘧啶。最后，为了促进体内碱基编辑，我们将我们的 sgBE 系统扩展到 AAV 兼容的 Cas9、SaCas9（金黄色葡萄球菌），并观察稳健的碱基编辑。