Obesity is characterized by low-grade chronic inflammation and an excess of adipose tissue. The ASC gene encodes a protein that is part of the NLRP3 inflammasome, a cytosolic multiprotein complex that is associated with inflammation and metabolic alterations. To our knowledge, there is no evidence regarding ASC gene activity in obese adults in response to lifestyle modifications. To evaluate the effect of hypocaloric diet and moderate-intensity structured exercise intervention on ASC gene expression and inflammatory markers in obese adults. Thirty-seven obese individuals aged 25 to 50 years were randomized to the hypocaloric diet exercise group or hypocaloric diet group. The participants underwent a 4-month follow-up. Electrical bioimpedance was used for body composition analysis. Biochemical data were analyzed by dry chemistry and insulin levels by ELISA. ASC gene expression from peripheral blood was performed using real-time PCR. Dietary data was collected through questionnaires and analyzed using the Nutritionist Pro™ software. Quantification of cytokines was conducted using Bio-Plex Pro™ Human cytokine. The Astrand-Ryhming test was used to estimate the maximum oxygen volume and design the moderate-intensity structured exercise program ~ 75% heart rate (HR) After the intervention, both study groups significantly improved body composition (decreased weight, fat mass, waist circumference and abdominal obesity, p < 0.05). Besides, the diet-exercise group significantly decreased ASC mRNA expression, MCP-1, and MIP-1β inflammatory cytokines compared to the diet group (p < 0.05). While in the diet group, MCP-1 and IL-8 exhibited significantly decreased levels (p < 0.05). In the diet-exercise group, a positive correlation between the atherogenic index and waist circumference was found (r = 0.822, p = 0.011), and a negative correlation was observed between the delta of ASC mRNA expression and IL-10 levels at the end of the intervention (r = − 0.627, p = 0.019). Low-grade chronic inflammation was attenuated through individualized exercise prescription and our findings highlight the role of the ASC gene in the inflammation of obese adults. ClinicalTrials.gov , number NCT04315376 . Registered 20 March 2020—retrospectively registered

中文翻译：

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一项肥胖研究表明，肥胖成年人的运动训练可通过降低外周血中ASC基因的含量来减轻低度的慢性炎症：一项前瞻性研究。

肥胖症的特征在于低度的慢性炎症和过量的脂肪组织。ASC基因编码的蛋白质是NLRP3炎性小体的一部分，NLRP3炎性小体是一种与炎症和代谢改变有关的胞质多蛋白复合物。据我们所知，没有证据表明肥胖成年人响应生活方式的改变而具有ASC基因活性。评估低热量饮食和中等强度的结构化运动干预对肥胖成年人ASC基因表达和炎性标志物的影响。37名25至50岁的肥胖者被随机分为低热量饮食运动组或低热量饮食组。参与者进行了为期4个月的随访。电生物阻抗用于身体成分分析。通过干化学分析生化数据，通过ELISA分析胰岛素水平。使用实时PCR从外周血中表达ASC基因。通过问卷调查收集膳食数据，并使用Nutritionist Pro™软件进行分析。细胞因子的定量是使用Bio-Plex Pro™人类细胞因子进行的。Astrand-Ryhming测试用于估计最大氧气量并设计中等强度的结构化运动计划，心率（HR）约为75％。干预后，两个研究组均显着改善了身体成分（体重，脂肪量，腰围减少）和腹部肥胖，p <0.05）。此外，饮食运动组与饮食组相比，ASC mRNA表达，MC​​P-1和MIP-1β炎性细胞因子显着降低（p <0.05）。在饮食组中 MCP-1和IL-8表现出明显降低的水平（p <0.05）。在饮食锻炼组中，发现动脉粥样硬化指数与腰围呈正相关（r = 0.822，p = 0.011），而在结束时ASC mRNA表达的变化与IL-10水平呈负相关。干预时间（r = − 0.627，p = 0.019）。低度慢性炎症通过个性化的运动处方得到缓解，我们的发现突出了ASC基因在肥胖成人炎症中的作用。ClinicalTrials.gov，编号NCT04315376。于2020年3月20日注册-追溯注册 干预结束时，ASC mRNA表达的变化量与IL-10水平呈负相关（r = − 0.627，p = 0.019）。低度慢性炎症通过个性化的运动处方得到缓解，我们的发现突出了ASC基因在肥胖成人炎症中的作用。ClinicalTrials.gov，编号NCT04315376。于2020年3月20日注册-追溯注册 干预结束时，ASC mRNA表达的变化量与IL-10水平呈负相关（r = − 0.627，p = 0.019）。低度慢性炎症通过个性化的运动处方得到缓解，我们的发现突出了ASC基因在肥胖成人炎症中的作用。ClinicalTrials.gov，编号NCT04315376。于2020年3月20日注册-追溯注册