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Microsatellite variation revealed panmictic pattern for Triatoma brasiliensis (Triatominae: Reduviidae) in rural northeastern Brazil: the control measures implications.
BMC Genetics Pub Date : 2020-08-27 , DOI: 10.1186/s12863-020-00903-w
Claudia Mendonça Bezerra 1, 2 , Carlota Josefovicz Belisário 3 , Grasielle Caldas D'Ávilla Pessoa 4 , Aline Cristine Luiz Rosa 3 , Carla Patrícia Barezani 3 , Flávio Campos Ferreira 3 , Alberto Novaes Ramos 1 , Ricardo Esteban Gürtler 5, 6 , Liléia Diotaiuti 2
Affiliation  

Triatoma brasiliensis Neiva, 1911 is the main vector of Trypanosoma cruzi in the caatinga of Northeastern Brazil. Despite of its epidemiological relevance, there are few studies on its genetic variability. Using microsatellite markers, we characterized the variability and dynamics of infestation and reinfestation of T. brasiliensis after residual insecticide spraying in five surveys conducted in a well-defined rural area located in the municipality of Tauá, Ceará, between 2009 and 2015. We evaluated: (1) general variability among local of captures; (2) variability along the time analysis (2009, 2010 and 2015); (3) and reinfestation process. On the analysis (1) global and pairwise FST values suggested absence of clusters among the area. AMOVA indicated that total variation is mainly represented by individual differences. Absence of clustering indicates a panmitic unit, with free gene flow. For (2), Pairwise FST indicated alterations in the genetic profile of the triatomines along the time. (3) Analysis of the reinfestation process showed that the domiciliary units investigated had different sources of infestation despite of its proximity. Observed homogeneity can be explained by the great dispersal capacity of T. brasiliensis, overlapping the different environments. Persistent house infestation in Tauá may be attributed to the occurrence of postspraying residual foci and the invasion of triatomines from their natural habitats.

中文翻译:

微卫星变异揭示了巴西东北部农村地区巴西三角藻(Triatominae:Reduviidae)的恐慌模式:控制措施的意义。

巴西Triatoma brasiliensis Neiva,1911年是巴西东北部caatinga中的锥虫锥虫的主要媒介。尽管具有流行病学意义,但对其遗传变异性的研究很少。我们使用微卫星标记,在2009年至2015年之间,在塞阿拉瓦州陶阿市的一个定义明确的农村地区进行的五项调查中,对残留杀虫剂喷洒后巴西T.brasensis的侵染和再侵染的变化和动力学进行了表征。我们评估了: (1)捕获地点之间的普遍差异;(2)随时间变化的可变性(2009年,2010年和2015年);(3)和复生过程。在分析中(1),全球FST值和成对FST值表明该区域中不存在群集。AMOVA指出,总变化主要由个体差异表示。缺乏聚类表明是具有自由基因流的泛素单位。对于(2),成对的FST指示了随着时间的推移,三atomines的遗传特征发生了变化。(3)对再感染过程的分析表明,所调查的住所单位尽管距离很近,但感染来源却不同。观察到的同质性可以用巴西T. brasiliensis的巨大分散能力来解释,重叠在不同的环境中。陶阿州持续的房屋侵扰可能归因于祈祷后残留病源的发生和来自其自然栖息地的三氢嘧啶的入侵。(3)对再感染过程的分析表明,所调查的住所单位尽管距离很近,但感染来源却不同。观察到的同质性可以用巴西T. brasiliensis的巨大分散能力来解释,重叠在不同的环境中。陶阿州持续的房屋侵扰可能归因于祈祷后残留病源的发生和来自其自然栖息地的三氢嘧啶的入侵。(3)对再感染过程的分析表明,所调查的住所单位尽管距离很近,但感染来源却不同。观察到的同质性可以用巴西T. brasiliensis的巨大分散能力来解释,重叠在不同的环境中。陶阿州持续的房屋侵扰可能归因于祈祷后残留病源的发生和来自其自然栖息地的三氢嘧啶的入侵。
更新日期:2020-08-28
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