Stroke induces a robust inflammatory response. However, it still lacks a systematic view of the various immune cell types due to the limited numbers of fluorophore used in the traditional FACS technique. In our current study, we utilized the novel technique mass cytometry (CyTOF) to analyze multiple immune cell types. We detected these immune cells from the ischemic brain, peripheral blood, spleen, and bone marrow at different time courses after stroke. Our data showed (1) dynamic changes in the immune cell numbers in the ischemic brain and peripheral organs. (2) The expression levels of cell surface markers indicate the inflammation response status after stroke. Interestingly, CD62L, a key adhesion molecule, regulates the migration of leukocytes from blood vessels into secondary lymphoid tissues and peripheral tissues. (3) A strong leukocyte network across the brain and peripheral immune organs was identified using the R program at day 1 after ischemia, suggesting that the peripheral immune cells dramatically migrated into the ischemic areas after stroke. This study provides a systematic, wide view of the immune components in the brain and peripheral organs for a deep understanding of the immune response after ischemic stroke.

中文翻译：

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使用CyTOF技术对缺血性卒中后免疫成分的系统研究。

中风引起强烈的炎症反应。但是，由于在传统的FACS技术中使用的荧光团数量有限，因此对于各种免疫细胞类型仍然缺乏系统的了解。在我们当前的研究中，我们利用了新型技术细胞计数法（CyTOF）来分析多种免疫细胞类型。我们在中风后的不同时间段从缺血性脑，外周血，脾脏和骨髓中检测到了这些免疫细胞。我们的数据显示（1）缺血性脑和外周器官中免疫细胞数量的动态变化。（2）细胞表面标志物的表达水平指示中风后的炎症反应状态。有趣的是，关键的粘附分子CD62L调节白细胞从血管向次级淋巴组织和周围组织的迁移。（3）在缺血后第1天使用R程序在大脑和周围免疫器官中发现了强大的白细胞网络，这表明中风后外周免疫细胞急剧迁移到缺血区域。这项研究为大脑和周围器官的免疫成分提供了系统的，广泛的视野，以深入了解缺血性中风后的免疫反应。