Okanin is a major flavonoid found in Coreopsis tinctoria Nutt., arousing huge interest recently for its considerable biological characteristics including antioxidant, antineurotoxic, and antidiabetic activities. An ultrahigh performance liquid chromatography triple-quadrupole tandem mass spectrometry (UPLC-MS) was successfully used to determine okanin in rat plasma after oral administration of okanin. Bavachalcone acted as an internal standard (IS). By gradient elution, IS and analyte were separated on a C₁₈ column for 7 min at a flow rate of 0.25 mL/min with acetonitrile-0.1% acetic acid mobile phase. The stability, matrix effect, extraction recovery, accuracy, precision, linearity, and selectivity of the method were firstly demonstrated. The major pharmacokinetic parameters of okanin in rat plasma were then measured using the developed UPLC-MS method. An UPLC-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was finally established to obtain the specific and accurate mass of okanin in rat plasma after oral administration, and its proposed fragmentation was further elaborated.

中文翻译：

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超高效液相色谱-三重串联串联质谱法测定大鼠血浆中的欧肯宁及其药代动力学。

欧卡宁（Okanin）是在金鸡菊中发现的一种主要类黄酮，最近因其重要的生物学特性（包括抗氧化剂，抗神经毒药和抗糖尿病活性）而引起人们的极大兴趣。超高效液相色谱三重串联四极杆串联质谱（UPLC-MS）已成功地用于口服口服卵磷脂后测定大鼠血浆中的卵磷脂。Bavachalcone充当内标（IS）。通过梯度洗脱，IS和分析物在C ₁₈上分离乙腈-0.1％乙酸流动相，以0.25 mL / min的流速离心7分钟。首先证明了该方法的稳定性，基质效应，提取回收率，准确度，精密度，线性和选择性。然后使用发达的UPLC-MS方法测定大鼠血浆中okanin的主要药代动力学参数。最终建立了UPLC-四极杆飞行时间质谱（UPLC-Q-TOF-MS），以在口服后获得大鼠血浆中特定的和准确的okain质量，并进一步完善了其拟议的片段化方法。