Environmental DNA barcoding technology is gaining innovative applications. The effectiveness of current DNA barcode reference libraries in identifying amphipod barcodes and/or strengthening the existing library was tested. From 2500 amphipod individuals we barcoded 22 amphipod species belonging to 17 genera, 13 families among which 13 species were first time barcoded. More than 80 percent of the species were new distributional records. The minimum and maximum inter-specific pair-wise distance values was respectively 0.16 and 5.51 percent. Defining family specific species threshold values would be imperative, rather than expecting a universal barcode gap for amphipod species. The overall mean pair-wise distance, nucleotide diversity and Tajimas statistics were 3.59 percent, 0.27 and 2.62, respectively. There is a strong need to increase the number of amphipod species barcodes in the reference database. For better facilitation of environmental monitoring, the datasets could be exclusively accessed at BOLD through http://dx.doi.org/10.5883/DS-MAOI.

中文翻译：

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加强海洋两栖动物DNA条形码库以进行环境监测

环境DNA条形码技术正在获得创新应用。测试了当前DNA条形码参考文库在识别两栖动物条形码和/或增强现有文库中的有效性。从2500个双足纲个体中，我们对属于17个属的22个双足纲物种进行了条形码编码，其中有13个科，其中13种首次被条形码化。超过80％的物种是新的分布记录。种间的最小和最大成对距离值分别为0.16％和5.51％。必须确定家庭特定物种的阈值，而不是期望两栖动物物种具有通用的条形码缺口。总体平均成对距离，核苷酸多样性和塔吉玛斯统计分别为3.59％，0.27和2.62。强烈需要增加参考数据库中两栖动物种类条形码的数量。为了更好地促进环境监测，可以通过http://dx.doi.org/10.5883/DS-MAOI在BOLD独家访问数据集。