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L-Asparaginase activity analysis, ansZ gene identification and anticancer activity of a new Bacillus subtilis isolated from sponges of the Red Sea.
Bioscience, Biotechnology, and Biochemistry ( IF 1.4 ) Pub Date : 2020-08-28 , DOI: 10.1080/09168451.2020.1807310
Fuad Ameen 1 , Wafa A Alshehri 2 , Nouf M Al-Enazi 3 , Abobakr Almansob 1
Affiliation  

ABSTRACT

This study describes the isolation of various marine bacteriafrom sponges collected from the Red Sea (Saudi Arabia) andL-asparaginase (anti-cancer enzyme) production from bacterialisolates. The 16S rDNA based phylogenetic analysis revealed thatthe isolate WSA3 was a Bacillus subtilis. Its partial-length genesequence was submitted to GenBank under the accession numberMK072695. The new B. subtilis strain harbored the exact size(1128 bp) of the new L-asparaginase (ansZ) gene as confirmedby PCR and in gel visualization, which was submitted to the NCBIdatabase (accession number MN566442). The molecular weightof partially purified L-asparaginase was determined as 45 kDa bySDS-PAGE. In addition, the enzyme L-asparaginase did not showglutaminase activity which is very important from a medical pointof view. Moreover, 100 μg/mL of the partially purified B. subtilis Lasparaginaseshowed promising anti-cancer activities when testedagainst three cancer cell lines (HCT-116, MCF-7, and HepG2).



中文翻译:

从红海海绵分离的新枯草芽孢杆菌的L-天冬酰胺酶活性分析,ansZ基因鉴定和抗癌活性。

摘要

这项研究描述了从红海(沙特阿拉伯)收集的海绵中分离各种海洋细菌,以及从细菌分离物中生产L-天冬酰胺酶(抗癌酶)的方法。基于16S rDNA的系统发育分析表明分离株WSA3是枯草芽孢杆菌。其部分全长基因​​序列已提交给GenBank,登录号为MK072695。经PCR和凝胶可视化确认,新的枯草芽孢杆菌菌株具有新的L-天冬酰胺酶(ansZ)基因的确切大小(1128 bp),并已提交给NCBI数据库(登录号MN566442)。通过SDS-PAGE测定部分纯化的L-天冬酰胺酶的分子量为45kDa。另外,酶L-天冬酰胺酶没有显示谷氨酰胺酶活性,这从医学的观点来看是非常重要的。此外,部分纯化的B为100μg/ mL。

更新日期:2020-11-05
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