ABSTRACT

This study describes the isolation of various marine bacteriafrom sponges collected from the Red Sea (Saudi Arabia) andL-asparaginase (anti-cancer enzyme) production from bacterialisolates. The 16S rDNA based phylogenetic analysis revealed thatthe isolate WSA3 was a Bacillus subtilis. Its partial-length genesequence was submitted to GenBank under the accession numberMK072695. The new B. subtilis strain harbored the exact size(1128 bp) of the new L-asparaginase (ansZ) gene as confirmedby PCR and in gel visualization, which was submitted to the NCBIdatabase (accession number MN566442). The molecular weightof partially purified L-asparaginase was determined as 45 kDa bySDS-PAGE. In addition, the enzyme L-asparaginase did not showglutaminase activity which is very important from a medical pointof view. Moreover, 100 μg/mL of the partially purified B. subtilis Lasparaginaseshowed promising anti-cancer activities when testedagainst three cancer cell lines (HCT-116, MCF-7, and HepG2).

摘要

这项研究描述了从红海（沙特阿拉伯）收集的海绵中分离各种海洋细菌，以及从细菌分离物中生产L-天冬酰胺酶（抗癌酶）的方法。基于16S rDNA的系统发育分析表明分离株WSA3是枯草芽孢杆菌。其部分全长基因​​序列已提交给GenBank，登录号为MK072695。经PCR和凝胶可视化确认，新的枯草芽孢杆菌菌株具有新的L-天冬酰胺酶（ansZ）基因的确切大小（1128 bp），并已提交给NCBI数据库（登录号MN566442）。通过SDS-PAGE测定部分纯化的L-天冬酰胺酶的分子量为45kDa。另外，酶L-天冬酰胺酶没有显示谷氨酰胺酶活性，这从医学的观点来看是非常重要的。此外，部分纯化的B为100μg/ mL。