Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae),Insect Science

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Knockdown of specific cuticular proteins analogous to peritrophin 3 genes disrupt larval and ovarian development in Bactrocera dorsalis (Diptera: Tephritidae)
Insect Science ( IF 2.9 ) Pub Date : 2020-08-27 , DOI: 10.1111/1744-7917.12869
Qiu-Li Hou _{1,

2} , Er-Hu Chen ₁ , Wei Dou ₁ , Jin-Jun Wang ₁

Affiliation

Cuticular proteins (CPs) are critical components of the insect cuticle and play important roles in maintaining normal insect development and defense against various environmental stresses. The oriental fruit fly (Bactrocera dorsalis) is one of the most destructive pests worldwide, and its eight CPs analogous to peritrophin 3 (BdCPAP3) family genes have been identified in our previous study. In the present study, we further explored the possible roles of CPAP3 genes in B. dorsalis development. Each sequence of BdCPAP3 genes contained three conserved ChtBD2 (chitin-binding) domains. Spatial and temporal expression patterns revealed that the four BdCPAP3 genes (BdCPAP3-A1, B, E, and E2) might play important roles in larval pupariation of B. dorsalis. Moreover, treatment with a juvenile hormone analog (methoprene) significantly restricted expression of these four CPAP3 genes, whereas treatment with 20-hydroxy-ecdysone induced expression. The RNA interference (RNAi) results revealed that down-regulated CPAP3 genes led to significant delay of pupariation, and injection of dsBdCPAP3-E into 5-d-old B. dorsalis larvae caused approximately 40% mortality. Interestingly, we also confirmed that BdCPAP3-D2 was involved in B. dorsalis ovarian development. This study showed that some specific CPAP3 genes had crucial roles in B. dorsalis development, and these CP genes could be used as potential targets to control this pest via RNAi.

中文翻译：

敲除类似于 peritrophin 3 基因的特定表皮蛋白会破坏 Bactrocera dorsalis（双翅目：实蝇科）的幼虫和卵巢发育

角质层蛋白 (CPs) 是昆虫角质层的重要组成部分，在维持昆虫正常发育和抵御各种环境压力方面发挥着重要作用。东方果蝇（Bactrocera dorsalis）是世界范围内最具破坏性的害虫之一，在我们之前的研究中已经确定了它的 8 个类似于 peritrophin 3 ( BdCPAP3 ) 家族基因的CP 。在本研究中，我们进一步探讨了CPAP3基因在B. dorsalis发育中的可能作用。BdCPAP3基因的每个序列都包含三个保守的 ChtBD2（几丁质结合）域。时空表达模式显示四种BdCPAP3基因（BdCPAP3-A1、B、E和E2 ) 可能在B. dorsalis 的幼虫化蛹中起重要作用。此外，用保幼激素类似物（methoprene）处理显着限制了这四种 CPAP3 基因的表达，而用 20-羟基蜕皮激素处理则诱导表达。RNA 干扰 (RNAi) 结果显示下调CPAP3基因导致蛹的显着延迟，将 dsBdCPAP3-E 注射到 5 日龄B. dorsalis幼虫中导致大约 40% 的死亡率。有趣的是，我们还证实BdCPAP3-D2参与了B. dorsalis卵巢发育。该研究表明，某些特定的CPAP3基因在B. dorsalis发育中起着至关重要的作用，这些 CP 基因可用作通过 RNAi 控制这种害虫的潜在目标。

更新日期：2020-08-27

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