Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2020-08-28 , DOI: 10.1016/j.hpj.2020.08.006 Haochun Xi , Yongjun He , Huoying Chen
Anthocyanin is abundant in a few vegetables, including eggplant. It protects plants from abiotic stress and benefits human health, making the research of anthocyanin biosynthesis increasingly important. Flowering time is an important reference for judging reproduction and adaptability, which can guide plant production. In this study, SmbHLH13 from eggplant was identified. Yeast one-hybrid and dual-luciferase assays showed that SmbHLH13 binded and activated the expression of structural genes SmCHS and SmF3H in anthocyanin biosynthesis and it also was bound to the promoter of the key gene SmFT in flowering. Furthermore, genetic transformation of Arabidopsis revealed that overexpression of SmbHLH13 enhanced anthocyanin accumulation and delayed flowering. These results demonstrated that SmbHLH13 might promote anthocyanin accumulation through positive regulation of SmCHS and SmF3H. Moreover, SmbHLH13 might have a role in delaying eggplant flowering.
中文翻译:
SmbHLH13在茄子花色苷生物合成和开花中的功能表征
花色苷在包括茄子在内的一些蔬菜中含量丰富。它保护植物免受非生物胁迫并有益于人类健康,这使得花色苷生物合成的研究日益重要。开花时间是判断繁殖力和适应性的重要参考,可以指导植物的生产。在这项研究中,鉴定了来自茄子的SmbHLH13。酵母单杂交和双萤光素酶测定显示SmbHLH13绑定和激活结构基因的表达SmCHS和SmF3H在花青素合成,它也被结合到关键基因的启动子SMFT开花。此外,拟南芥的遗传转化揭示SmbHLH13的过表达增强了花色苷的积累并延迟了开花。这些结果表明,SmbHLH13可能推动通过的正调控花青素积累SmCHS和SmF3H。此外,SmbHLH13可能在延迟茄子开花中起作用。