Hydrogen sulfide (H₂S) is a very important third endogenously generated gaseous signaling molecule and plays a key role in physiological and pathological regulation processes of living biosystems. Although a lot of H₂S fluorescent probes have been reported, the relationship between the physiology and pathology of H₂S in inflamed tissues remains unclear. Herein, by adopting a donor-π-acceptor (D-π-A)-structured naphthalimide derivative as the two-photon (TP) fluorophore and a 4-dinitrobenzene-ether (DNB) with a strong intramolecular charge transfer (ICT) effect as the recognition moiety, we reported a novel TP bioimaging probe NP-H₂S for H₂S with improved sensitivity. The NP-H₂S exhibits very low background fluorescence in the absence of H₂S, and a significant 258-fold fluorescence intensity enhancement was observed in the presence of H₂S, resulting in a high sensitivity and selectivity to H₂S in aqueous solutions with a detection limit of 18.8 nM observed. The probe also shows a wide linear response concentration range (0–10.0 μM) to H₂S with high selectivity. All these features are favorable for direct monitoring of H₂S in complex biological samples. It was then applied for direct TP imaging of H₂S in tissues of inflammation model with satisfactory sensitivity, indicating it has the latent capability in further biological applications for investigation of the interaction H₂S with inflammation.

硫化氢（H ₂ S）是一种非常重要的第三种内源性产生的气态信号分子，在生物系统的生理和病理调节过程中起着关键作用。尽管已经报道了许多H ₂ S荧光探针，但是发炎组织中H ₂ S的生理学和病理学之间的关系仍然不清楚。本文中，通过采用供体-π-受体（D-π-A）结构的萘二甲酰亚胺衍生物作为双光子（TP）荧光团和4-二硝基苯-醚（DNB），具有很强的分子内电荷转移（ICT）作用。作为识别部分，我们报道了一种新型的TP生物成像探针NP - H ₂ S用于H ₂具有更高的灵敏度。的NP - ħ ₂小号表现出在不存在H的非常低的背景荧光₂ S，和在H的存在下观察到一个显著258倍的荧光强度增强₂ S，从而导致高的灵敏度和选择性成H ₂ S IN观察到的检测极限为18.8 nM的水溶液。该探针还显示出对H ₂ S的宽线性响应浓度范围（0–10.0μM）和高选择性。所有这些功能都直接监视H的有利₂复杂生物样品S中。然后将其用于H _2的直接TP成像炎症模型组织中的S具有令人满意的敏感性，表明它在研究H ₂ S与炎症相互作用的其他生物学应用中具有潜在能力。