Pandanus tectorius fruit, a natural product rich in tangeretin and ethyl caffeate, has been reported to have potential as anti-hypercholesterolemia agent via Scavenger Receptor Class B type 1 (SR-B1) pathway. However, due to its semi-polar properties, P. tectorius extract exhibits poor solubility when used as a medical remedy. The extract’s solubility can potentially be improved through a synthesis of nanoparticles of chitosan-P. tectorius fruit extract. This can also increase the extract’s SR-B1 gene expression activity. To date, no studies of nanoparticles of chitosan-P. tectorius fruit extract and its pathway via SR-B1 have been published anywhere. In this study, cytotoxicity properties against HepG2 were explored by MTT. Then luciferase assay was used to detect their effectiveness in increasing SR-B1 activity. An in vivo study using Sprague dawley was carried out to observe the extract nanoparticles’ effectiveness in reducing the cholesterol levels and the toxicity property in rat’s liver. As the results showed, the extract nanoparticles had no cytotoxic activity against HepG2 cells and exhibited higher SR-B1 gene expression activity than the non-nanoparticle form. As the in vivo study proved, nanoparticle treatment can reduce the levels of TC (197%), LDL (360%), and TG (109%), as well as increase the level of HDL cholesterol by 150%, in comparison to those for the untreated high-cholesterol diet group. From the toxicity study, it was found that there was non-toxicity in the liver. It can be concluded that nanoparticles of chitosan-P. tectorius fruit extract successfully increased P. tectorius fruit extract’s effectiveness in reducing hypercholesterolemia via SR-B1 pathway. Hence, it can be suggested that nanoparticles of chitosan-P. tectorius fruit extract is safe and suitable as an alternative treatment for controlling hypercholesterolemia via SR-B1 pathway.

据报道，露兜果是一种富含橘子和咖啡酸乙酯的天然产物，可通过清道夫受体 B 型 1 (SR-B1) 途径作为抗高胆固醇血症药物。然而，由于其半极性特性，当用作医疗药物时， P. tectorius提取物表现出较差的溶解度。提取物的溶解度可以通过合成壳聚糖-P. tectorius果实提取物的纳米颗粒来提高。这也可以增加提取物的 SR-B1 基因表达活性。迄今为止，没有关于壳聚糖-P. tectorius纳米粒子的研究水果提取物及其通过 SR-B1 的途径已在任何地方发表。在这项研究中，MTT 探索了针对 HepG2 的细胞毒性特性。然后使用荧光素酶测定来检测它们在增加 SR-B1 活性方面​​的有效性。使用Sprague dawley进行了一项体内研究，以观察提取物纳米颗粒在降低大鼠肝脏中胆固醇水平和毒性方面的有效性。结果表明，提取物纳米颗粒对HepG2细胞没有细胞毒活性，并且比非纳米颗粒形式表现出更高的SR-B1基因表达活性。作为体内研-胆固醇饮食组。从毒性研究中发现，对肝脏没有毒性。可以得出结论，壳聚糖-毛茛果实提取物纳米颗粒通过SR-B1途径成功地提高了毛茛果实提取物降低高胆固醇血症的有效性。因此，可以表明壳聚糖-P. tectorius果实提取物的纳米颗粒是安全的，适合作为通过 SR-B1 途径控制高胆固醇血症的替代疗法。