Abstract Amphiphilic alkyl-peptides as novel biomaterials form 3D scaffolds that are applicable in tissue engineering. Here, the nanofibre formation capability of a distinct alkyl-peptide was investigated using coarse-grained molecular dynamics simulation (CGMD) and experimental methods. The alkyl-peptide (Ace-FAQRVPPEEEGGGAAAAK-Nhe(C16)) was functionalized with a peptide epitope (FAQRVPPP) which can help to maintenance and differentiation of neural stem cells. Two alkyl-peptide systems were investigated: the all-functionalized system (with only bioactive alkyl-peptides) and the distributed system (a combination of bioactive and non-bioactive alkyl-peptides with ratio 1:2). The CGMD and TEM results confirm elongated nanofibres for all-functionalized system and cylindrical nanofibres for the distributed one. Furthermore, PC12 cells show a reliable growth on both 2D alkyl-peptides coated surfaces. Because of the nanofibres negative surface charges, the cell morphologies show clustered form in the distributed system and rounded shape in the all-functionalized one. Since the stem cell state preserves in cluster form, the physicochemical property of these nanofibres allows a potential advantage in stem cell long-time maintains.

中文翻译：

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功能化烷基肽在纳米纤维形成和神经祖细胞活力和存活中的理化性质作用

摘要 作为新型生物材料的两亲性烷基肽形成了适用于组织工程的 3D 支架。在这里，使用粗粒度分子动力学模拟（CGMD）和实验方法研究了不同烷基肽的纳米纤维形成能力。烷基肽 (Ace-FAQRVPPEEEGGGAAAAK-Nhe(C16)) 被肽表位 (FAQRVPPP) 功能化，有助于神经干细胞的维持和分化。研究了两种烷基肽系统：全功能化系统（只有生物活性烷基肽）和分布式系统（生物活性和非生物活性烷基肽的组合，比例为 1:2）。CGMD 和 TEM 结果证实了全功能化系统的细长纳米纤维和分布式系统的圆柱形纳米纤维。此外，PC12 细胞在两个二维烷基肽包被的表面上均显示出可靠的生长。由于纳米纤维表面带负电荷，细胞形态在分布式系统中呈簇状，在全功能化系统中呈圆形。由于干细胞状态以簇形式保存，这些纳米纤维的物理化学特性允许长期维持干细胞的潜在优势。