Detection of cysteine is crucial as its deviation from normal concentration leads to various diseases. In this work, benzothiazole based chemodosimeter was synthesized to discriminate cysteine rapidly in the presence of other competing interferences. The probe exhibit ESIPT governed colorimetric and fluoremetric response on addition of cysteine via conjugate addition-cyclization reaction and it is confirmed by NMR and HRMS. The probe can detect lower concentration of cysteine with limit of detection 0.07 μM in 2 min that assist the system to work well in biological systems. Hence the sensor was profitably employed in imaging of cysteine in HeLa cell line and variance in the redox state was also investigated.

半胱氨酸的检测至关重要，因为其偏离正常浓度会导致各种疾病。在这项工作中，基于苯并噻唑的化学计量仪被合成为在存在其他竞争性干扰的情况下快速区分半胱氨酸。该探针在通过共轭加成环化反应添加半胱氨酸后表现出ESIPT控制的比色和荧光响应，并通过NMR和HRMS证实。该探针可在2分钟内检测到较低浓度的半胱氨酸，检测限为0.07μM，有助于该系统在生物系统中正常工作。因此，该传感器被有利地用于HeLa细胞系中半胱氨酸的成像，并且还研究了氧化还原状态的变化。