Mitochondrial genomes of the three lucanid species in the Dorcus velutinus complex – Dorcus velutinus Thomson, D. ursulus Arrow and D. tenuihirsutus Kim and Kim – were assembled and analyzed through next generation sequencing. The mitogenome sequences were used to infer phylogenetic relationships among Dorcus species. Our analyses revealed that the newly sequenced mitogenomes are comparable in their size, content, and gene arrangement to other lucanid mitogenomes reported to date. However, we confirmed the presence of a large intergenic spacer (IGS) between trnS^(UCN) and ND1 genes, whose length varied from 170 bp (in D. tenuihirsutus) to 193 bp (in D. ursulus and D. velutinus). Within this IGS region, a short sequence fragment (TACTAAATT) was found uniquely across the three species of Dorcus velutinus complex. Our phylogenetic analyses show that the D. velutinus complex constitutes a distinct clade with a significant divergence from other species of the genus Dorcus sensu stricto. Furthermore, we reaffirm the validity of D. tenuihirsutus – a species originally described from Korea – as a distinct species, though the taxonomic status of D. ursulus remains to be studied further. Finally, we find the presence and location of large IGSs to be useful for studying evolutionary history and species delimitation in stag beetles.

在三个lucanid品种的线粒体基因组多克斯蝠-复杂多克斯蝠汤姆森D. ursulus箭头和D. tenuihirsutus Kim和金-组装，并通过新一代测序分析。有丝分裂基因组序列被用来推断Dorcus物种之间的系统发生关系。我们的分析表明，新测序的有丝分裂基因组在大小，内容和基因安排方面与迄今报道的其他lucanid有丝分裂基因组具有可比性。但是，我们证实了trnS ^（UCN）和ND1基因之间存在一个较大的基因间隔区（IGS），其长度从170 bp（在D. tenuihirsutus）到193 bp（在D. ursulus和D. velutinus中）。在该IGS区域内，发现唯一的短序列片段（TACTAAATT）跨越了Dorcus velutinus复合体的三种物种。我们的系统发育分析表明，D。velutinus复合物构成了一个独特的进化枝，与其他Dorcus sensu stricto菌种有显着差异。此外，我们重申D. tenuihirsutu s（最初从韩国描述的物种）作为不同物种的有效性，尽管D. ursulus的分类地位有待进一步研究。最后，我们发现大型IGS的存在和位置对于研究锹形甲虫的进化历史和物种界定非常有用。