Patient derived xenograft (PDX) models provide an efficient way to study anti-tumor drug efficacy. In this respect, it is essential to study the optimal method needed to cryopreserve the starting cells obtained from tumor samples for PDX model generation. Cryopreservation of cells prior to xenografting is necessary for cross-verification of results obtained by xenografting and also for practical planning of experiments. In the present work, we studied the cryopreservation of colorectal carcinoma (CRC) cells isolated from patient tumor samples for generating their patient derived xenograft models. CRC therapeutics study is essential for early stage intervention and treatment of the disease. CRC cell lines do not ideally depict the molecular characteristics of patient CRC tumor samples. This necessitates the generation of CRC PDX models for drug discovery. We show that CRC cells isolated from patient tumor samples have comparable recovery, viability and growth with both conventional cryopreservation methods as well as Fibulas BioFlash Drive™. However, xenograft tumor formation was much more effective with Fibulas BioFlash Drive™ cryopreserved cells than with cells cryopreserved with conventional methods. Therefore, we put forward an effective way to cryopreserve primary cells obtained from patient tumor samples for PDX model generation in this study.

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一种冷冻保存结直肠癌细胞用于患者来源异种移植模型生成的新方法

患者衍生的异种移植 (PDX) 模型提供了一种研究抗肿瘤药物功效的有效方法。在这方面，必须研究冷冻保存从肿瘤样本中获得的起始细胞以用于 PDX 模型生成所需的最佳方法。异种移植前细胞的冷冻保存对于异种移植获得的结果的交叉验证以及实验的实际计划是必要的。在目前的工作中，我们研究了从患者肿瘤样本中分离的结直肠癌 (CRC) 细胞的冷冻保存，以生成其患者衍生的异种移植模型。CRC 治疗学研究对于疾病的早期干预和治疗至关重要。CRC 细胞系不能理想地描述患者 CRC 肿瘤样本的分子特征。这需要为药物发现生成 CRC PDX 模型。我们表明，从患者肿瘤样本中分离的 CRC 细胞具有与传统冷冻保存方法和 Fibulas BioFlash Drive™ 相当的恢复、活力和生长。然而，使用 Fibulas BioFlash Drive™ 冷冻保存的细胞比使用传统方法冷冻保存的细胞更有效地形成异种移植肿瘤。因此，我们在本研究中提出了一种有效的方法来冷冻保存从患者肿瘤样本中获得的原代细胞以用于 PDX 模型生成。与使用传统方法冷冻保存的细胞相比，使用 Fibulas BioFlash Drive™ 冷冻保存的细胞形成异种移植肿瘤的效果要好得多。因此，我们在本研究中提出了一种有效的方法来冷冻保存从患者肿瘤样本中获得的原代细胞以用于 PDX 模型生成。与使用传统方法冷冻保存的细胞相比，使用 Fibulas BioFlash Drive™ 冷冻保存的细胞形成异种移植肿瘤的效果要好得多。因此，我们在本研究中提出了一种有效的方法来冷冻保存从患者肿瘤样本中获得的原代细胞以用于 PDX 模型生成。