The DNA repair system has evolved from the common ancestor of all life forms and its function is highly conserved within eukaryotes. In this study, to reveal the role of DNA double-strand break repair (DSB) genes in response to benzo[α]pyrene (B[α]P), we first identified DSB genes in relation to homologous recombination and non-homologous end joining events in four Brachionus rotifer spp.: B. calyciflorus, B. koreanus, B. plicatilis, and B. rotundiformis. In all the Brachionus spp., 39 orthologous genes to human DSB repair genes were identified. Furthermore, three genes in B. koreanus, two genes in B. plicatilis, and one gene in B. calyciflorus and B. rotundiformis were present as duplicated genes, indicating that these genes were diversified over speciation in the genus Brachionus. Moreover, we compared DSB repair genes on the gene structures in four monogonont Brachionus rotifers and the bdelloid rotifer Adineta vaga, which possesses highly efficient DNA repair ability. The transcriptional responses of four monogonont Brachionus rotifers in response to B[α]P exposure showed how B[α]P exposure led to DSBs and subsequently recruited DNA DSB repair pathways in the rotifer B. koreanus. Taken together, this study provides a better understanding of the potential role of DSB repair genes in the monogonont rotifer Brachionus spp. in response to B[α]P.

DNA修复系统已经从所有生命形式的共同祖先进化而来，其功能在真核生物中高度保守。在这项研究中，为了揭示DNA双链断裂修复（DSB）基因在响应苯并[α] py（B [α] P）中的作用，我们首先鉴定了与同源重组和非同源末端有关的DSB基因。四个Brachionus rotifer spp。中的连接事件：B. calyciflorus，B. koreanus，B. plicatilis和B. rotundiformis。在所有Brachionus物种中，鉴定出39种与人DSB修复基因同源的基因。此外，韩国芽孢杆菌中的三个基因，褶皱芽孢杆菌中的两个基因和朝鲜蓟马中的一个基因。花萼芽孢杆菌和轮状芽孢杆菌作为重复的基因存在，表明这些基因在Brachionus属中由于物种的形成而多样化。此外，我们比较了四种monogonont基因结构DSB修复基因臂轮虫和蛭形轮虫Adineta瓦加，它具有高效的DNA修复能力。四个monogonont的转录反应臂轮虫响应于B [α]芘曝光表明乙如何[α] p曝光导致DNA双链断裂和在随后轮虫招募DNA DSB修复途径B.朝鲜球。两者合计，这项研究提供了更好的理解DSB修复基因在轮虫轮枝Brachionus spp中的潜在作用。响应B [α] P。