Abstract

A non-extraction method for determining the concentration of chlorosomal bacteriochlorophylls (Bchl), photosynthetic pigments of green sulfur bacteria has been developed and tested. The method is based on the measurement of optical density spectra of microorganisms directly in natural water and is aimed at simplifying Bchl concentration measurements, reducing the measurement time and eliminating toxic solvents from the experiments. Comparison of the results of Bchl concentration determination by the traditional method based on the measurement of absorption spectra of pigment extracts and the new method that is based on the measurement of long wavelength absorption band of Bchl in bacterial cells in natural water showed a high correlation coefficient from 0.9866 to 0.9991 for different series of samples. The depth distribution profiles of Bchl concentration obtained from the absorption spectra of natural water in some meromictic reservoirs of the White Sea region in September 2019 and Lake Mogilnoye (Barents Sea) in June 2019 are presented.

中文翻译：

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利用天然水中绿色硫细菌的吸收光谱定量测定绿藻中的细菌叶绿素

摘要

已经开发和测试了一种非萃取方法，用于测定绿硫细菌的光合色素-绿体细菌叶绿素（Bchl）的浓度。该方法基于直接在天然水中测量微生物的光密度光谱，旨在简化Bchl浓度测量，减少测量时间并从实验中消除有毒溶剂。传统方法（基于色素提取物的吸收光谱的测定）与新方法（基于Bchl在自然水中细菌细胞中长波长吸收带的测定）的Bchl浓度测定结果的比较显示出较高的相关系数从0.9866到0.9991，适用于不同系列的样品。