Tissue-specific fat deposition is regulated by a series of complex regulatory mechanisms. Reports indicate that epigenetic regulators, such as circular RNAs (circRNAs), are crucial in diseases progression, animal development, metabolism, and adipogenesis. In this study, to assess the functional roles of circRNAs in adipogenesis and tissue-specific fat deposition, we comprehensively analyzed the Ribo-Zero RNA-Seq and miRNAs data during chicken intramuscular and abdominal adipogenic differentiation. circRNAs and miRNAs profiles during chicken adipogenic differentiation were found in adipocytes derived from various adipose tissues. It was also discovered that high levels of downregulated miRNAs potentially promote adipogenesis by activating their target genes which are associated with fatty acid metabolism and adipogenic differentiation. Through analysis of the correlation between the expression levels of circRNAs and adipogenic genes, as well as the dynamic expression patterns of circRNAs during adipogenic differentiation, several candidate circRNAs were identified. Moreover, competing endogenous RNA (ceRNAs) networks were constructed during chicken intramuscular and abdominal adipogenesis by combining miRNAs with mRNAs data. Several candidate circRNAs potentially influence adipogenesis by regulating miRNAs via PPAR and fatty acid metabolism-related pathways were identified, such as circLCLAT1, circFNDC3AL, circCLEC19A and circARMH1. In conclusion, our findings reveal that circRNAs and the circRNA-miRNAs-mRNAs-ceRNAs network may play important roles in chicken adipocytes differentiation and tissue-specific fat deposition.

中文翻译：

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对circRNA，miRNA和mRNA谱进行综合分析，以揭示鸡肌肉和腹部脂肪形成中的ceRNA网络。

组织特异性脂肪沉积受一系列复杂的调控机制调控。报告表明，表观遗传调节剂，例如环状RNA（circRNA），在疾病进展，动物发育，代谢和脂肪形成中至关重要。在这项研究中，为评估circRNA在脂肪形成和组织特异性脂肪沉积中的功能，我们在鸡的肌肉和腹部脂肪形成分化过程中全面分析了Ribo-Zero RNA-Seq和miRNA数据。在源自各种脂肪组织的脂肪细胞中发现了成鸡分化过程中的circRNA和miRNA谱。还发现高水平下调的miRNA可能通过激活其与脂肪酸代谢和成脂分化有关的靶基因而促进成脂。通过分析circRNA和成脂基因表达水平之间的相关性，以及成脂分化过程中circRNA的动态表达模式，鉴定了几种候选circRNA。此外，在鸡的肌肉和腹部脂肪形成过程中，通过将miRNA与mRNA数据相结合，构建了竞争性内源RNA（ceRNA）网络。几种候选circRNA可能通过通过PPAR调节miRNA和与脂肪酸代谢相关的途径来调控miRNA，从而潜在地影响脂肪形成，例如circLCLAT1，circFNDC3AL，circCLEC19A和circARMH1。总之，我们的发现表明，circRNA和circRNA-miRNA-mRNA-ceRNAs网络可能在鸡脂肪细胞分化和组织特异性脂肪沉积中起重要作用。