当前位置：
X-MOL 学术
›
J. Mater. Chem. B
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A thermoresponsive gel photoreleasing nitric oxide for potential ocular applications.
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-08-27 , DOI: 10.1039/d0tb01194k Mimimorena Seggio 1 , André Luiz Tessaro 2 , Antonia Nostro 3 , Giovanna Ginestra 3 , Adriana C E Graziano 4 , Venera Cardile 4 , Stefano Acierno 5 , Pietro Russo 6 , Ovidio Catanzano 7 , Fabiana Quaglia 7 , Salvatore Sortino 1
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-08-27 , DOI: 10.1039/d0tb01194k Mimimorena Seggio 1 , André Luiz Tessaro 2 , Antonia Nostro 3 , Giovanna Ginestra 3 , Adriana C E Graziano 4 , Venera Cardile 4 , Stefano Acierno 5 , Pietro Russo 6 , Ovidio Catanzano 7 , Fabiana Quaglia 7 , Salvatore Sortino 1
Affiliation
|
We report herein the design, preparation, characterization and biological evaluation of a thermoresponsive gel based on binary mixtures of Pluronic® co-polymers F127 and P123, the latter being covalently functionalized with a nitric oxide (NO) photodonor (NOPD). The weight ratio between the two polymeric components is optimized in order to observe gelation of their saline water solution in the range of 32–35 °C, in order to exploit the therapeutic properties of NO for potential ocular applications. Rheological measurements were performed to evaluate the gelation temperature and, hence, to select a co-polymer mixture specifically appropriate for the reference application. Integration of the NOPD into the polymeric scaffold does not affect its rheological and spectroscopic properties, making it a good absorber of visible light both in solution and in the gel phase. Irradiation of the saline solution of the polymeric components with visible light triggers NO release, which occurs with an efficiency of more than one order of magnitude faster than that observed for the isolated NOPD. The polymeric system fully preserves such photobehavior after gelation as demonstrated by the effective NO photorelease from the gel matrix and its diffusion in the supernatant upon illumination. The gel is well-tolerated in both dark and light conditions by corneal cells, while being able to induce growth inhibition towards Staphylococcus aureus under visible light irradiation and has high moduli which can contribute to an adequate retention time within the eyes.
中文翻译：
一种热敏凝胶光释放一氧化氮，用于潜在的眼部应用。
我们在此报告了基于 Pluronic® 共聚物 F127 和 P123 的二元混合物的热敏凝胶的设计、制备、表征和生物学评估，后者用一氧化氮 (NO) 光电供体 (NOPD) 进行共价功能化。对两种聚合物组分之间的重量比进行优化，以便观察其盐水溶液在 32-35 °C 范围内的凝胶化，从而利用 NO 的治疗特性用于潜在的眼部应用。进行流变学测量以评估胶凝温度，从而选择特别适合参考应用的共聚物混合物。将 NOPD 整合到聚合物支架中不会影响其流变学和光谱特性，使其成为溶液和凝胶相中可见光的良好吸收剂。用可见光照射聚合物组分的盐溶液会触发 NO 释放，其发生效率比分离的 NOPD 观察到的效率快一个数量级以上。凝胶化后，聚合物体系完全保留了这种光行为，这一点可以从凝胶基质中有效的 NO 光释放及其在光照下在上清液中的扩散来证明。该凝胶在黑暗和光照条件下均能被角膜细胞良好耐受，同时能够在可见光照射下诱导金黄色葡萄球菌的生长抑制，并且具有高模量，有助于在眼睛内有足够的保留时间。
更新日期：2020-10-14
中文翻译：
一种热敏凝胶光释放一氧化氮，用于潜在的眼部应用。
我们在此报告了基于 Pluronic® 共聚物 F127 和 P123 的二元混合物的热敏凝胶的设计、制备、表征和生物学评估，后者用一氧化氮 (NO) 光电供体 (NOPD) 进行共价功能化。对两种聚合物组分之间的重量比进行优化，以便观察其盐水溶液在 32-35 °C 范围内的凝胶化，从而利用 NO 的治疗特性用于潜在的眼部应用。进行流变学测量以评估胶凝温度，从而选择特别适合参考应用的共聚物混合物。将 NOPD 整合到聚合物支架中不会影响其流变学和光谱特性，使其成为溶液和凝胶相中可见光的良好吸收剂。用可见光照射聚合物组分的盐溶液会触发 NO 释放，其发生效率比分离的 NOPD 观察到的效率快一个数量级以上。凝胶化后，聚合物体系完全保留了这种光行为，这一点可以从凝胶基质中有效的 NO 光释放及其在光照下在上清液中的扩散来证明。该凝胶在黑暗和光照条件下均能被角膜细胞良好耐受，同时能够在可见光照射下诱导金黄色葡萄球菌的生长抑制，并且具有高模量，有助于在眼睛内有足够的保留时间。
京公网安备 11010802027423号