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Electrogenerated chemiluminescence resonance energy transfer between ZnGa2O4/g-C3N4 and gold nanoparticles/graphene and its application in the detection of thrombin.
Analyst ( IF 3.6 ) Pub Date : 2020-08-26 , DOI: 10.1039/d0an01558j Hui Liu 1 , Hao Yin , TingTing Yang , HouCheng Ding , YongPing Dong
Analyst ( IF 3.6 ) Pub Date : 2020-08-26 , DOI: 10.1039/d0an01558j Hui Liu 1 , Hao Yin , TingTing Yang , HouCheng Ding , YongPing Dong
Affiliation
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In the present work, ZnGa2O4 was incorporated with g-C3N4 nanosheets to synthesize ZnGa2O4/g-C3N4 nanocomposites through the hydrothermal method. The morphologies of nanocomposites were characterized by TEM, XRD, and spectral and electrochemical methods, respectively. The nanocomposites exhibited greatly enhanced fluorescence with the maximum emission peak red-shifted from 380 nm to 450 nm. A strong cathodic electrogenerated chemiluminescence (ECL) signal of ZnGa2O4/g-C3N4 nanocomposites was obtained under neutral conditions, which was much stronger than those of pure materials. ECL resonance energy transfer (ECL-RET) occurred between ZnGa2O4/g-C3N4 and gold nanoparticle/graphene nanocomposites, resulting in an apparent decrease of the ECL signal. Based on this, a label-free ECL sensor for thrombin was fabricated. The sensor showed high sensitivity, wide linearity, and good selectivity for the detection of thrombin in the range from 1.37 fM to 27.4 pM with a detection limit of 0.55 fM (3σ). The proposed method was applied to detect thrombin in serum samples with satisfactory results. This work revealed a new role of spinel-type semiconductor oxide nanomaterials, which will provide more ECL systems for the fabrication of biosensors.
中文翻译：
ZnGa2O4 / g-C3N4与金纳米颗粒/石墨烯之间的电化学发光共振能量转移及其在凝血酶检测中的应用。
在目前的工作中，ZnGa 2 O 4与gC 3 N 4纳米片结合在一起，通过水热法合成ZnGa 2 O 4 / gC 3 N 4纳米复合材料。纳米复合材料的形貌分别用TEM，XRD，光谱和电化学方法表征。纳米复合材料显示出大大增强的荧光，最大发射峰从380 nm移至450 nm。ZnGa 2 O 4 / gC 3 N 4的强阴极电致化学发光（ECL）信号纳米复合材料是在中性条件下获得的，它比纯材料强得多。ECL共振能量转移（ECL-RET）在ZnGa 2 O 4 / gC 3 N 4与金纳米颗粒/石墨烯纳米复合材料之间发生，导致ECL信号明显降低。基于此，制造了用于凝血酶的无标记ECL传感器。该传感器在1.37 fM至27.4 pM的范围内对凝血酶的检测显示出高灵敏度，宽线性度和良好的选择性，检测极限为0.55 fM（3σ）。该方法用于血清中凝血酶的检测，结果令人满意。这项工作揭示了尖晶石型半导体氧化物纳米材料的新作用，它将为制造生物传感器提供更多的ECL系统。
更新日期：2020-09-20
中文翻译：
ZnGa2O4 / g-C3N4与金纳米颗粒/石墨烯之间的电化学发光共振能量转移及其在凝血酶检测中的应用。
在目前的工作中，ZnGa 2 O 4与gC 3 N 4纳米片结合在一起，通过水热法合成ZnGa 2 O 4 / gC 3 N 4纳米复合材料。纳米复合材料的形貌分别用TEM，XRD，光谱和电化学方法表征。纳米复合材料显示出大大增强的荧光，最大发射峰从380 nm移至450 nm。ZnGa 2 O 4 / gC 3 N 4的强阴极电致化学发光（ECL）信号纳米复合材料是在中性条件下获得的，它比纯材料强得多。ECL共振能量转移（ECL-RET）在ZnGa 2 O 4 / gC 3 N 4与金纳米颗粒/石墨烯纳米复合材料之间发生，导致ECL信号明显降低。基于此，制造了用于凝血酶的无标记ECL传感器。该传感器在1.37 fM至27.4 pM的范围内对凝血酶的检测显示出高灵敏度，宽线性度和良好的选择性，检测极限为0.55 fM（3σ）。该方法用于血清中凝血酶的检测，结果令人满意。这项工作揭示了尖晶石型半导体氧化物纳米材料的新作用，它将为制造生物传感器提供更多的ECL系统。
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