Abstract The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera; Aleyrodidae), and greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae), are highly problematic plant pests and virus vectors with worldwide distributions. Identification of whitefly species is typically accomplished by observation of distinct morphological characters; however, because of morphological inconsistency and indistinguishability, the discrimination of B. tabaci species variants is dependent on molecular techniques based on genetic differences. New assays were designed for the detection of B. tabaci A, B, and Q mitotype groups, and T. vaporariorum. Specific primer sets were designed for amplification of the mitochondrial cytochrome c oxidase I gene of the four targets to perform in end-point PCR, real-time PCR coupled to high-resolution melting analysis (HRM), and the isothermal helicase-dependent amplification (HDA). Primer specificities were validated using end-point PCR, then tested in HRM and HDA. Bemisia tabaci A, B, and Q mitotypes, and T. vaporariorumtargeted primer sets discriminately amplified specimens of different populations within their target whitefly group. These tests provide three novel discrimination assays for the high-consequence, exotic B. tabaci B and Q groups, along with the native B. tabaci A group and T. vaporariorum.

中文翻译：

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探索使用高分辨率熔解分析和解旋酶相关扩增来鉴别烟粉虱（半翅目：粉虱科）隐性物种和汽绿粉虱

摘要 甘薯粉虱 Bemisia tabaci (Gennadius) (Hemiptera; Aleyrodidae) 和温室粉虱 Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) 是问题严重的植物害虫和病毒载体，分布于世界各地。粉虱种类的鉴定通常通过观察不同的形态特征来完成；然而，由于形态上的不一致和不可区分性，烟粉虱物种变体的区分取决于基于遗传差异的分子技术。设计了新的检测方法来检测烟粉虱 A、B 和 Q 有丝分裂群，以及烟粉虱。设计了特定的引物组，用于扩增四个靶标的线粒体细胞色素 c 氧化酶 I 基因，以在终点 PCR 中进行，实时 PCR 结合高分辨率熔解分析 (HRM) 和等温解旋酶依赖性扩增 (HDA)。使用终点 PCR 验证引物特异性，然后在 HRM 和 HDA 中进行测试。Bemisia tabaci A、B 和 Q 有丝分裂型，以及 T.vaporariorum 靶向引物组在其目标粉虱组中区分扩增不同种群的标本。这些测试为后果严重的异国烟粉虱 B 和 Q 组，以及本地烟粉虱 A 组和汽烟尘肺提供了三种新颖的鉴别分析。Vaporariorum 靶向引物组在其目标粉虱组中区分扩增不同种群的标本。这些测试为后果严重的异国烟粉虱 B 和 Q 组，以及本地烟粉虱 A 组和汽烟尘肺提供了三种新颖的鉴别分析。Vaporariorum 靶向引物组在其目标粉虱组中区分扩增不同种群的标本。这些测试为后果严重的异国烟粉虱 B 和 Q 组，以及本地烟粉虱 A 组和汽烟尘肺提供了三种新颖的鉴别分析。