Dynamics of Ionic Interactions at Protein-Nucleic Acid Interfaces.,Accounts of Chemical Research

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Dynamics of Ionic Interactions at Protein-Nucleic Acid Interfaces.
Accounts of Chemical Research ( IF 16.4 ) Pub Date : 2020-08-26 , DOI: 10.1021/acs.accounts.0c00212
Binhan Yu ₁ , B Montgomery Pettitt ₁ , Junji Iwahara ₁

Affiliation

Molecular association of proteins with nucleic acids is required for many biological processes essential to life. Electrostatic interactions via ion pairs (salt bridges) of nucleic acid phosphates and protein side chains are crucial for proteins to bind to DNA or RNA. Counterions around the macromolecules are also key constituents for the thermodynamics of protein–nucleic acid association. Until recently, there had been only a limited amount of experiment-based information about how ions and ionic moieties behave in biological macromolecular processes. In the past decade, there has been significant progress in quantitative experimental research on ionic interactions with nucleic acids and their complexes with proteins. The highly negatively charged surfaces of DNA and RNA electrostatically attract and condense cations, creating a zone called the ion atmosphere. Recent experimental studies were able to examine and validate theoretical models on ions and their mobility and interactions with macromolecules. The ionic interactions are highly dynamic. The counterions rapidly diffuse within the ion atmosphere. Some of the ions are released from the ion atmosphere when proteins bind to nucleic acids, balancing the charge via intermolecular ion pairs of positively charged side chains and negatively charged backbone phosphates. Previously, the release of counterions had been implicated indirectly by the salt-concentration dependence of the association constant.

中文翻译：

蛋白质-核酸界面离子相互作用的动力学。

蛋白质与核酸的分子缔合是生命必不可少的许多生物学过程所必需的。通过核酸磷酸根离子对（盐桥）和蛋白质侧链的静电相互作用对于蛋白质与DNA或RNA的结合至关重要。大分子周围的抗衡离子也是蛋白质-核酸缔合热力学的关键成分。直到最近，关于离子和离子部分在生物大分子过程中的行为方式的基于实验的信息仍然非常有限。在过去的十年中，在与核酸的离子相互作用及其与蛋白质的复合物的定量实验研究中取得了重大进展。DNA和RNA高度带负电的表面会静电吸引和凝聚阳离子，创建一个称为离子气氛的区域。最近的实验研究能够检查和验证有关离子及其迁移率以及与大分子相互作用的理论模型。离子相互作用是高度动态的。抗衡离子在离子气氛中迅速扩散。当蛋白质与核酸结合时，一些离子会从离子气氛中释放出来，通过带正电的侧链和带负电的主链磷酸酯的分子间离子对来平衡电荷。以前，缔合常数的盐浓度依赖性间接牵涉抗衡离子的释放。抗衡离子在离子气氛中迅速扩散。当蛋白质与核酸结合时，一些离子会从离子气氛中释放出来，通过带正电的侧链和带负电的主链磷酸酯的分子间离子对来平衡电荷。以前，缔合常数的盐浓度依赖性间接牵涉抗衡离子的释放。抗衡离子在离子气氛中迅速扩散。当蛋白质与核酸结合时，一些离子会从离子气氛中释放出来，通过带正电的侧链和带负电的主链磷酸酯的分子间离子对来平衡电荷。以前，缔合常数的盐浓度依赖性间接牵涉抗衡离子的释放。

更新日期：2020-09-15

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