Abstract

In this study, phthalocianato[bis(dimethylaminoethanoxy)] silicon (NzPC) was loaded onto gelatin nanoparticles functionalized with polyelectrolytes (polystyrene sulfonate/polyallylamine hydrochloride) by layer-by-layer (LbL) assembly for photodynamic therapy (PDT) application in promastigote form of Leishmania amazonensis treatment. The process yield, and encapsulation efficiency were 80.0% ± 1.8 and EE = 87.0% ± 1.1, respectively. The polyelectrolytic gelatin nanoparticles (PGN) had a mean diameter of 437.4 ± 72.85 nm, narrow distribution size with a polydispersity index of 0.086. The obvious switching of zeta potential indicates successful alternating deposition of the polyanion PSS and polycation PAH directly on the gelatin nanoparticles. Photosensitizer photophysical properties were shown to be preserved after gelatin nanoparticle encapsulation. The impact of the PDT in the viability and morphology of Leishmania amazonensis promastigote in culture medium was evaluated. The PGN-NzPc presented low toxicity at the dark and the PDT was capable of decreasing the viability in more than 80% in 0.1 µmol.L⁻¹ concentration tested. The PDT also triggered significant morphological alterations in the Leishmania promastigotes. These results reinforce the idea that the use of PGN as photosensitizers carriers is useful for PDT of Leishmania promastigotes.

摘要

在这项研究中，酞菁[双（二甲氨基乙氧基）]硅（NzPC）通过逐层（LbL）组装加载到用聚电解质（聚苯乙烯磺酸盐/聚烯丙胺盐酸盐）功能化的明胶纳米颗粒上，用于前鞭毛体形式的光动力疗法（PDT）应用的利什曼原虫亚马孙治疗。工艺产量和封装效率分别为 80.0% ± 1.8 和 EE = 87.0% ± 1.1。聚电解质明胶纳米颗粒 (PGN) 的平均直径为 437.4 ± 72.85 nm，分布尺寸窄，多分散指数为 0.086。zeta 电位的明显转换表明聚阴离子 PSS 和聚阳离子 PAH 直接交替沉积在明胶纳米颗粒上。明胶纳米颗粒封装后显示出光敏剂光物理特性得以保留。评估了 PDT 对培养基中Leishmania amazonensis前鞭毛体的生存力和形态的影响。PGN-NzPc 在黑暗中表现出低毒性，PDT 能够在 0.1 µmol.L ^-1中降低 80% 以上的生存力浓度测试。PDT 还引发了利什曼原虫前鞭毛体的显着形态学改变。这些结果强化了使用 PGN 作为光敏剂载体对利什曼原虫前鞭毛体的PDT 有用的想法。