Abstract

Hairy root induction in Plantago lanceolata was optimized to take advantage of transformed root cultures. The highest frequency of transformation was achieved using leaf explant, A4 strain, pre-cultivation of explant, 150 µM Acetosyringone, 5 min inoculation, half-strength Murashige and Skoog basal medium as co-cultivation, and half-strength Gamborg′s basal medium as a selective medium with 3% sucrose. Among the studied compound encompassing gallic acid, catalpol and apigenin, only the production of gallic acid in hairy roots was affected by 20 mg L⁻¹ AgNO₃ and 100 mg L⁻¹ chitosan at 24 hr which yielded 7.63, 4.76-fold increase in its content, respectively. The methanolic extracts of hairy roots elicited by 20 mg L⁻¹ AgNO₃ exhibited anti-bacterial activity (MIC and MBC = 25 mg mL⁻¹) against Klebsiella pneumoniae, Proteus vulgaris and Salmonella typhi and anti-bacterial potential of non-elicited hairy roots of P. lanceolata (MIC = 25 mg mL⁻¹ and MBC = 35 mg mL⁻¹) were more active against Klebsiella pneumoniae and P. vulgaris than other bacteria. The methanolic extracts of the P. lanceolata hairy roots demonstrated significant cytotoxic activity on colorectal carcinoma cell line (SW-480) with IC₅₀ = 250.65 ± 6.8 µg mL⁻¹ in comparison to human embryonic kidney (HEK-293) with IC₅₀ = 5263.65 ± 4.6 µg mL⁻¹. Plantago lanceolata hairy roots showed important biological activity explaining its role in traditional medicine.

抽象的

优化了车前草中毛状根的诱导，以利用转化的根培养物。使用叶片外植体，A4菌株，外植体预培养，150 µM乙酰丁香酮，5分钟接种，半强度Murashige和Skoog基础培养基作为共同培养以及半强度Gamborg的基础培养基可实现最高的转化频率作为含3％蔗糖的选择性培养基。在所研究的化合物中，包括没食子酸，catalpol和芹菜素，在24小时内仅毛状根中没食子酸的产生受到20 mg L ^-1 AgNO ₃和100 mg L ^-1壳聚糖的影响，产生7.63倍，是4.76倍。其内容。20 mg L ^-1诱发毛状根的甲醇提取物AgNO ₃表现出对肺炎克雷伯菌，寻常变形杆菌和鼠伤寒沙门氏菌的抗菌活性（MIC和MBC = 25 mg mL ^-1），以及未引起的轮叶假单胞菌毛状根的抗菌潜力（MIC = 25 mg mL ^-1）和MBC = 35 mg mL ^-1）比其他细菌对肺炎克雷伯菌和寻常毕赤酵母的活性更高。轮叶毛茸茸根的甲醇提取物对大肠癌细胞系（SW-480）表现出显着的细胞毒性活性，IC ₅₀ = 250.65±6.8 µg mL ^-1与人胚肾（HEK-293）相比，IC ₅₀ = 5263.65±4.6 µg mL ^-1。车前草毛状根显示出重要的生物学活性，这解释了其在传统医学中的作用。