Abstract Inspired by mussel-adhesion phenomenon in wet environment, polydopamine (PDA) was tried in recent research reports to improve the dentin adhesive interface with better strength and durability. The aim of this study was to comparatively evaluate the effect of PDA incorporated dentin adhesive systems and conventional adhesive systems on degree of conversion (DC), resin dentin bond strength (SBS), durability and MMP inhibition activity. In this study, PDA was incorporated in total-etch adhesive system (TE) and self-etch adhesive systems (SE) and degree of conversion (DC%) was evaluated using Fourier transform infrared spectroscopy. Dentin slabs obtained from 160 non-carious human premolars were divided into the following: Group 1, TE; Group 2, PDA incorporated TE adhesive (TEP); Group 3, SE; Group 4, PDA incorporated SE adhesive (SEP) and bonding procedures were performed. Samples were stored for either 24 h or 6 months, after which shear bond strength and resin dentin interface was evaluated using universal testing machine and confocal laser scanning microscopy (CLSM), respectively. The zymographic assay was performed on protein extracts obtained from dentin powder treated with each of the adhesives or with or without PDA followed by functional activity assay for MMP-2 and -9. Statistical analysis was done with two-way analysis of variance and Student t-test at significance level as p < 0.05. There was statistically no significant difference in the DC% between PDA incorporated adhesives and control. No significant reduction in SBS was observed in TEP and SEP in pre- and post-aging while CLSM showed better penetration depth of resin and lesser degradation of hybrid layer on aging. Significantly lesser MMP activity was seen in PDA incorporated adhesives qualitatively and quantitatively on zymographic and functional MMP activity assay respectively. HIGHLIGHTS Catechol moieties present in polydopamine provide anti-collagenolytic activity, MMP inhibitory activity and enhances wet adhesion. Polydopamine incorporated dentin bonding systems attempts to provide stable resin dentin bond with increased bond durability. It can inhibit both proteolytic and hydrolytic breakdown of resin–dentin bond degradation.

中文翻译：

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含聚多巴胺的牙本质粘合剂对粘接耐久性的影响

摘要 受潮湿环境中贻贝黏附现象的启发，近期研究报告尝试使用聚多巴胺（PDA）来改善牙本质黏附界面，使其具有更好的强度和耐久性。本研究的目的是比较评估加入 PDA 的牙本质粘合剂系统和传统粘合剂系统对转化度 (DC)、树脂牙本质粘合强度 (SBS)、耐久性和 MMP 抑制活性的影响。在这项研究中，PDA 被纳入全蚀刻粘合剂系统 (TE) 和自蚀刻粘合剂系统 (SE)，并使用傅里叶变换红外光谱评估转化程度 (DC%)。从 160 颗非龋人类前磨牙获得的牙本质板分为以下几类：第 1 组，TE；第 2 组，PDA 并入 TE 粘合剂 (TEP)；第 3 组，东南；第 4 组，进行了加入 PDA 的 SE 粘合剂 (SEP) 和粘合程序。样品储存 24 小时或 6 个月，之后分别使用万能试验机和共聚焦激光扫描显微镜 (CLSM) 评估剪切粘合强度和树脂牙本质界面。对从用每种粘合剂或用或不用 PDA 处理的牙本质粉末获得的蛋白质提取物进行酶谱分析，然后对 MMP-2 和 -9 进行功能活性分析。统计分析是通过方差的双向分析和学生 t 检验在显着性水平上进行的，p < 0.05。加入PDA的粘合剂和对照之间的DC%在统计学上没有显着差异。在老化前后的 TEP 和 SEP 中没有观察到 SBS 的显着降低，而 CLSM 显示出更好的树脂渗透深度和老化时混合层的较少降解。分别在酶谱和功能性 MMP 活性测定中定性和定量地观察到 PDA 掺入的粘合剂中显着较低的 MMP 活性。亮点聚多巴胺中存在的儿茶酚部分提供抗胶原蛋白分解活性、MMP 抑制活性并增强湿粘附。包含聚多巴胺的牙本质粘接系统试图提供稳定的树脂牙本质粘接，并具有更高的粘接耐久性。它可以抑制树脂-牙本质键降解的蛋白水解和水解分解。分别在酶谱和功能性 MMP 活性测定中定性和定量地观察到 PDA 掺入的粘合剂中显着较低的 MMP 活性。亮点聚多巴胺中存在的儿茶酚部分提供抗胶原蛋白溶解活性、MMP 抑制活性并增强湿粘附。包含聚多巴胺的牙本质粘接系统试图提供稳定的树脂牙本质粘接，并具有更高的粘接耐久性。它可以抑制树脂-牙本质键降解的蛋白水解和水解分解。分别在酶谱和功能性 MMP 活性测定中定性和定量地观察到 PDA 掺入的粘合剂中显着较低的 MMP 活性。亮点聚多巴胺中存在的儿茶酚部分提供抗胶原蛋白溶解活性、MMP 抑制活性并增强湿粘附。包含聚多巴胺的牙本质粘接系统试图提供稳定的树脂牙本质粘接，并具有更高的粘接耐久性。它可以抑制树脂-牙本质键降解的蛋白水解和水解分解。