Pronuciferine is a naturally occurring proaporphine alkaloid that belongs to isoquinoline alkaloids. The aim of this study is to investigate the neuroactivity of pronuciferine. We assessed the neuroprotective effect of pronuciferine against hydrogen peroxide (H₂O₂)‐induced apoptosis in human neuronal SH‐SY5Y cells. In addition, we measured the effect of pronuciferine on cell metabolites and brain‐derived neurotrophic factor (BDNF) level in SH‐SY5Y cells. In vitro result shows that pronuciferine at 10 µM significantly (P < .001) increased the proliferation of SH‐SY5Y by 45%, and upon H₂O₂ addition, pronuciferine significantly (P < .001) suppressed neuronal death caused by H₂O₂. Gas Chromatography‐Mass Spectrometry (GC‐MS) metabolomics study revealed that pronuciferine has a high impact on glycine‐serine‐threonine pathway by changing the intracellular level of serine dimethylglycine, sarcosine, and threonine. Also, pronuciferine increased the intercellular level of aspartic acid, glutamine, and tryptophan. Additionally, pronuciferine significantly (P < .05) increased the intracellular BDNF protein expression at 10 µM. Therefore, pronuciferine is a neuroactive molecule that might act as a neuroprotective agent to prevent apoptosis in neurodegenerative diseases.

中文翻译：

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天然存在的proaporphine生物碱，pronuciferine的神经活性。

核苷元是属于异喹啉类生物碱的天然的proaporphine生物碱。这项研究的目的是研究核素的神经活性。我们评估了pronuciferine对过氧化氢（H ₂ O ₂）诱导的人神经元SH-SY5Y细胞凋亡的神经保护作用。此外，我们测量了前核苷对SH-SY5Y细胞中细胞代谢产物和脑源性神经营养因子（BDNF）水平的影响。体外结果显示，在10 µM的情况下，前核黄素显着（P  <.001）使SH‐SY5Y的增殖增加了45％，并且在添加H ₂ O _2后，前核黄素显着（P  <.001）抑制了由H引起的神经元死亡。₂ O ₂。气相色谱-质谱（GC-MS）代谢组学研究表明，pronuciferine通过改变丝氨酸二甲基甘氨酸，肌氨酸和苏氨酸的细胞内水平对甘氨酸-丝氨酸-苏氨酸途径有很大影响。另外，前核黄素增加了天冬氨酸，谷氨酰胺和色氨酸的细胞间水平。此外，pronuciferine 在10 µM时显着增加（P <.05）细胞内BDNF蛋白表达。因此，前核苷元是一种神经活性分子，可以作为神经保护剂来预防神经退行性疾病中的细胞凋亡。