Jasmonate is an essential phytohormone regulating plant growth, development, and defense. Alternative splicing (AS) in jasmonate ZIM‐domain (JAZ) repressors is well‐characterized and plays an important role in jasmonate signaling regulation. However, it is unknown whether other genes in the jasmonate signaling pathway are regulated by AS. We explore the potential for AS regulation in three Arabidopsis genotypes (WT, jaz2, jaz7) in response to methyl jasmonate (MeJA) treatment with respect to: (a) differential AS, (b) differential miRNA targeted AS, and (c) AS isoforms with novel functions. AS events identified from transcriptomic data were validated with proteomic data. Protein interaction networks identified two genes, SKIP and ALY4 whose products have both DNA‐ and RNA‐binding affinities, as potential key regulators mediating jasmonate signaling and AS regulation. We observed cases where AS alone, or AS and transcriptional regulation together, can influence gene expression in response to MeJA. Twenty‐one genes contain predicted miRNA target sites subjected to AS, which implies that AS is coupled to miRNA regulation. We identified 30 cases where alternatively spliced isoforms may have novel functions. For example, AS of bHLH160 generates an isoform without a basic domain, which may convert it from an activator to a repressor. Our study identified potential key regulators in AS regulation of jasmonate signaling pathway. These findings highlight the importance of AS regulation in the jasmonate signaling pathway, both alone and in collaboration with other regulators.

中文翻译：

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茉莉酸在拟南芥中诱导选择性剪接反应。


茉莉酸是调节植物生长、发育和防御的重要植物激素。茉莉酸 ZIM 结构域 (JAZ) 阻遏物中的选择性剪接 (AS) 已得到充分表征，并且在茉莉酸信号传导调节中发挥着重要作用。然而，目前尚不清楚茉莉酸信号通路中的其他基因是否受AS调节。我们探索了三种拟南芥基因型（WT、 jaz2 、 jaz7 ）响应茉莉酸甲酯（MeJA）处理的 AS 调节潜力，涉及：（a）差异性 AS，（b）差异性 miRNA 靶向 AS，以及（c）AS具有新颖功能的亚型。从转录组数据中鉴定出的 AS 事件用蛋白质组数据进行了验证。蛋白质相互作用网络确定了两个基因SKIP和ALY4 ，其产物具有 DNA 和 RNA 结合亲和力，作为介导茉莉酸信号传导和 AS 调节的潜在关键调节因子。我们观察到单独的 AS 或 AS 与转录调控一起可以影响 MeJA 反应的基因表达的情况。 21 个基因包含预测的受 AS 影响的 miRNA 靶位点，这意味着 AS 与 miRNA 调控相关。我们鉴定了 30 个案例，其中选择性剪接异构体可能具有新功能。例如， bHLH160的 AS 生成没有基本结构域的同种型，这可以将其从激活子转换为阻遏子。我们的研究确定了茉莉酸信号通路 AS 调节的潜在关键调节因子。这些发现强调了 AS 调节在茉莉酸信号通路中的重要性，无论是单独调节还是与其他调节器合作调节。