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A threshold dosage of estrogen for male-to-female sex reversal in the Glandirana rugosa frog.
Journal of Experimental Zoology Part A ( IF 1.9 ) Pub Date : 2020-08-26 , DOI: 10.1002/jez.2408
Akira Oike 1 , Yoriko Nakamura 2 , Shigeki Yasumasu 3 , Etsuro Ito 1 , Masahisa Nakamura 4
Affiliation  

Steroid hormones play very important roles in gonadal differentiation in many vertebrate species. Previously, we have determined a threshold dosage of testosterone (T) to induce female‐to‐male sex reversal in Glandirana rugosa frogs. Genetic females formed a mixture of testis and ovary, the so‐called ovotestis, when tadpoles of G. rugosa were reared in water containing the dosage of T, which enabled us to detect primary changes in the histology of the masculinizing gonads. In this study, we determined a threshold dosage of estradiol‐17β (E2) to cause male‐to‐female sex reversal in this frog. We observed first signs of histological changes in the ovotestes, when tadpoles were reared in water containing the dosage of E2. Ovotestes were significantly larger than wild‐type testes in size. By E2 treatment, male germ cells degenerated in the feminizing testis leading to their final disappearance. In parallel, oocytes appeared in the medulla of the ovotestis and later in the cortex as well. Quantitative polymerase chain reaction analysis revealed that the expression of sex‐related genes involved in testis formation was significantly decreased in the ovotestis. In addition, immuno‐positive signals of CYP17 that is involved in testis differentiation in this frog disappeared in the medulla first and then in the cortex. These results suggested that oocytes expanded in the feminizing gonad (ovary) contemporaneously with male germ cell disappearance. Primary changes in the histology of the gonads during male‐to‐female sex reversal occurred in the medulla and later in the cortex. This direction was opposite to that observed during female‐to‐male sex reversal in the G. rugosa frog.

中文翻译:

皱land蛙中雌雄性逆转的雌激素阈值剂量。

类固醇激素在许多脊椎动物物种的性腺分化中起着非常重要的作用。以前,我们已经确定了睾丸激素(T)的阈值剂量,以诱导皱眉蛙雌性至雄性逆转。遗传雌性形成睾丸和卵巢,所谓的卵睾的混合物,当蝌蚪G.藿香在含有T剂量的水中饲养,这使我们能够检测到男性化性腺的组织学的主要变化。在这项研究中,我们确定了引起这只青蛙雌雄性逆转的雌二醇-17β(E2)阈值剂量。当t在含有E2剂量的水中饲养时,我们观察到卵睾丸组织学变化的初步迹象。卵睾丸的大小明显大于野生型睾丸。通过E2处理,雄性生殖细胞在女性化的睾丸中退化,导致其最终消失。平行地,卵母细胞出现在卵睾丸的延髓中,随后也出现在皮质中。定量聚合酶链反应分析显示,卵睾丸中与睾丸形成有关的性相关基因的表达明显降低。此外,CYP17的免疫阳性信号与该青蛙的睾丸分化有关,首先在髓质中消失,然后在皮层中消失。这些结果表明,卵母细胞在雌性性腺(卵巢)同时扩张,而雄性生殖细胞消失。男女性别逆转期间性腺组织学的主要变化发生在延髓,随后发生在皮质。这个方向与女性性别逆转过程中观察到的方向相反。男女性别逆转期间性腺组织学的主要变化发生在延髓,随后发生在皮质。这个方向与女性性别逆转过程中观察到的方向相反。男女性别逆转期间性腺组织学的主要变化发生在延髓,随后发生在皮质。这个方向与女性性别逆转过程中观察到的方向相反。G. rugosa青蛙。
更新日期:2020-10-30
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