A rapid and sensitive ultrahigh‐performance liquid chromatography–tandem mass spectrometry method was developed and validated for simultaneous determination of 15 bioactive ingredients in rat plasma and tissues after oral administration of Polygonum chinense Linn extract (PCE). After addition of internal standards (ISs; rutin and danshensu), plasma and tissue samples were pre‐treated by protein precipitation with acetonitrile–ethanol. The chromatographic separation was performed on an Agilent ZORBAX RRHD Eclipse Plus C₁₈ column with gradient elution using a mobile phase composed of methanol and water (containing 0.2% acetic acid) at a flow rate of 0.3 mL min⁻¹. Mass spectrometric detection was carried out using a mass spectrometer in both positive and negative ion electrospray ionization modes by multiple reaction monitoring. The method provided excellent linearity, and the lower limit of quantification range 0.5–30 ng mL⁻¹ for all analytes. The intra‐ and inter‐day precision were less than 9.12% and the accuracy ranged from −4.02% to 6.32%, respectively. The mean extraction recovery and matrix effect of analytes and ISs ranged from 83.65% to 109.20%. The method was successfully applied to the pharmacokinetics and tissue distribution study of 15 ingredients of PCE in rats.

中文翻译：

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超高效液相色谱-串联质谱法研究虎杖提取物中15种成分的药代动力学和组织分布

建立了一种快速灵敏的超高效液相色谱-串联质谱方法，并经验证可用于口服虎Poly提取物（PCE）后同时测定大鼠血浆和组织中的15种生物活性成分。加入内标（ISs，芦丁和丹参素）后，血浆和组织样品通过乙腈-乙醇沉淀进行蛋白质预处理。色谱分离在Agilent ZORBAX RRHD Eclipse Plus C ₁₈色谱柱上进行，使用甲醇和水（含0.2％乙酸）组成的流动相，以0.3 mL min ^-1的流速进行梯度洗脱。使用质谱仪以正离子和负离子电喷雾电离模式通过多个反应监控进行质谱检测。该方法具有出色的线性，所有分析物的定量下限为0.5–30 ng mL ^-1。日内和日间准确度均小于9.12％，准确度分别为−4.02％至6.32％。分析物和IS的平均提取回收率和基质效应范围为83.65％至109.20％。该方法已成功地应用于15种PCE成分在大鼠体内的药代动力学和组织分布研究。